Lecture 16. Names and experiment

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mame727
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Lecture 16. Names and experiment
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2015-02-11 00:54:21
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Lecture 16
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Lecture 16. Names and Experiments
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  1. Who determined that DNA is the heritable material?

    What did their experiment involve?
    Frederick Griffith is the one who noted that DNA is the heritable material.

    Frederic Griffth's experiment involved the R strain being converted into the S strain.
  2. Who determined that DNA is the transforming agent?

    How did they do this?
    Avery, MacLeod and McCarty determined that DNA is the transforming agent.

    They destroyed polysaccharides, lipids, RNA, and proteins and all mice died. But when the DNA was destroyed, the mouse lived due to no live S strain being recovered.
  3. Who determined that the phage genetic material is the DNA?

    What radioactive isotopes did they add and where did they add them?
    Hershey and Chase determined that the phage genetic material is DNA.  

    They added the S35 to the T2 phage and they added the p32 to the DNA of the T2 phage.
  4. What is Chargaff's Rule?
    Chargaff's Rule is that the total number of pyrimidines= the total number of purines: (T+C)= (A+G) and A=T and C=G.
  5. What are Rosalind Franklin's critical experimental result?

    What is an Angstrom?

    What did Watson and Crick provide?
    Rosalind Franklin's critical experimental results were that DNA is a helix with a regular width of 34 Angstroms per turn and 3.4 Angstroms per base pair. 

    An Angstrom is 1*10^-10 meters.

    Watson and Crick provided the first model of DNA.
  6. What happens when you add a Pyrimidine and another Pyrimidine?

    What happens if you add two purines?

    What happens when you add a purine and a pyrimidine?

    Is caffeine a purine or a pyrimidine?
    When you add a Pyrimidine and another Pyrimidine, you have DNA that is too thin.

    When you add two purines, you get DNA that is too thick.

    When you add a purine and a pyrimidine, you get the thickness that is compatible with X-ray data.

    Caffeine is a purine.
  7. Is DNA replication conservative or semiconservative?

    Who determined that DNA is copied by semiconservative replication?
    DNA replication is semiconservative. 

    Meselson-Stahl is the one who determined that DNA is copied by a semiconservative replication.

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