Quiz 2

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Skitty2004
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296622
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Quiz 2
Updated:
2015-02-23 12:56:06
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biochem
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biochem2
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biochem
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  1. ATP synthesis in mitochondrion is driven by energy produced in electron 
    transport pathway. And this energy is stored in the form of ________. ATP synthesized in this process is called _____ hosphorylation. 
    ATP produced in TCA cycle is called ______ phosphorylation.
    • proton gradient
    • oxidative
    • substrate level
  2. Which of the following step does NOT produce NADH?
    Succinate ----> fumarate
  3. All the enzymes in the TCA cycle are water soluble and located in the matrix 
    of mitochondrion. True or False.
    False
  4. Pyruvate dehydrogenase is regulated by which covalent modification?
    phosphorylation
  5. The precise mechanism of NADH-UQ reductase is unknown, but the electron 
    transport involves...
    FMN
  6. Which complex is not coupled with proton translocation in electron 
    transport pathway _______.
    Compex II
  7. The components of electron transport chain include two mobile electron 
    carriers, a lipid-soluble molecule _________ and a water-soluble protein __________.
    • UQ
    • Cytochrome c
  8. Which of the following enzyme is not allosterically inhibited by high level of 
    NADH?
    Pyruvate carboxylase
  9. Please write down the coenzymes involved in the reaction: pyruvate -------> acetyl-CoA.
    FAD, CoA, Liopic Acid, TPP,NAD+/NADH
  10. The terminal electron acceptor in eukaryotic aerobes is _______.
    O2 (oxygen)
  11. If the F0 unit of ATP synthase has the stoichiometry of a1b2c12, How many 
    degrees of rotation of one of the c subunits relative to the a subunit occur 
    with each proton transferred to the matrix?
    30
  12. Which enzyme in the citric acid cycle proceeds by a similar mechanism and 
    with similar cofactors as pyruvate dehydrogenase?
    α-Ketoglutarate dehydrogenase
  13. Q cycle is a unique redox pathway in ______.
    Cytochrome bc1
  14. Which complex of the electron transport chain does not contain a 
    cytochrome?
    NADH-Coenzyme Q reductase
  15. The electron transfer in complex IV is from cytochrome c to _____ to 
    heme a, then to ________ and in the end to O2.
    • CuA
    • Heme a3/CuB
  16. The energy-requiring step of ATP synthesis in ATP synthase is the release of ATP. True or False.
    True
  17. Electron transport causes the proton translocation of protons across the 
    inner membrane of mitochondria. What compartment has a lower pH?
    intermembrane space
  18. The glycerophsophate shuttle and ________ shuttle feed the electrons from _______ into electron transport in mitochondrion.
    • malate-aspartate
    • NADH produced from glycolysis in cytosol
  19. In your own words, please describe the purpose of TCA cycle in two or three 
    sentences. Please be brief and you don’t have to go into details of the 
    reactions
    To oxidize Acetyl-CoA into CO2 and release the energy in the form of NADH
  20. Please draw the pathway how the electrons from NADH are transferred to 
    O2. Write down the full names of the complexes.
    NADH----> NADH-UQ reductase----> UQ----> UQ-cytochrome c reductase-----> cytochrome c ----> cytochrome c oxidase-----> O2
  21. Please write down the reaction(s) that produces GTP in TCA cycle. Include 
    the enzymes (coenzymes) and the structures of the substrates and products. 
    And write down an intermediate (structure/name) during catalysis.
  22. For each acetate oxidized, the net outcome of the TCA cycle is...
    two molecules of CO2, one ATP, 3 NADH and one FADH2

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