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ATP synthesis in mitochondrion is driven by energy produced in electron
transport pathway. And this energy is stored in the form of ________. ATP synthesized in this process is called _____ hosphorylation.
ATP produced in TCA cycle is called ______ phosphorylation.
- proton gradient
- substrate level
Which of the following step does NOT produce NADH?
Succinate ----> fumarate
All the enzymes in the TCA cycle are water soluble and located in the matrix
of mitochondrion. True or False.
Pyruvate dehydrogenase is regulated by which covalent modification?
The precise mechanism of NADH-UQ reductase is unknown, but the electron
Which complex is not coupled with proton translocation in electron
transport pathway _______.
The components of electron transport chain include two mobile electron
carriers, a lipid-soluble molecule _________ and a water-soluble protein __________.
Which of the following enzyme is not allosterically inhibited by high level of
Please write down the coenzymes involved in the reaction: pyruvate -------> acetyl-CoA.
FAD, CoA, Liopic Acid, TPP,NAD+/NADH
The terminal electron acceptor in eukaryotic aerobes is _______.
If the F0 unit of ATP synthase has the stoichiometry of a1b2c12, How many
degrees of rotation of one of the c subunits relative to the a subunit occur
with each proton transferred to the matrix?
Which enzyme in the citric acid cycle proceeds by a similar mechanism and
with similar cofactors as pyruvate dehydrogenase?
Q cycle is a unique redox pathway in ______.
Which complex of the electron transport chain does not contain a
NADH-Coenzyme Q reductase
The electron transfer in complex IV is from cytochrome c to _____ to
heme a, then to ________ and in the end to O2.
The energy-requiring step of ATP synthesis in ATP synthase is the release of ATP. True or False.
Electron transport causes the proton translocation of protons across the
inner membrane of mitochondria. What compartment has a lower pH?
The glycerophsophate shuttle and ________ shuttle feed the electrons from _______ into electron transport in mitochondrion.
- NADH produced from glycolysis in cytosol
In your own words, please describe the purpose of TCA cycle in two or three
sentences. Please be brief and you don’t have to go into details of the
To oxidize Acetyl-CoA into CO2 and release the energy in the form of NADH
Please draw the pathway how the electrons from NADH are transferred to
O2. Write down the full names of the complexes.
NADH----> NADH-UQ reductase----> UQ----> UQ-cytochrome c reductase-----> cytochrome c ----> cytochrome c oxidase-----> O2
Please write down the reaction(s) that produces GTP in TCA cycle. Include
the enzymes (coenzymes) and the structures of the substrates and products.
And write down an intermediate (structure/name) during catalysis.
For each acetate oxidized, the net outcome of the TCA cycle is...
two molecules of CO2, one ATP, 3 NADH and one FADH2
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