Culture Media

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corbin19
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29693
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Culture Media
Updated:
2010-08-12 20:52:22
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Culture Media Micro Microbiology NSHS MLT
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Culture Media 7.2
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  1. this type of media grows most non-fastidious organisms with no advantage given to a particular organism.
    supportive/general
  2. this type of media is enriched for fastidious organisms.
    enrichment
  3. this type of media is inhubitory.
    selective
  4. this type of media is used as a visual aid.
    differential
  5. this type of media is confirmatory ID for previously isolated organisms.
    Biochemical
  6. this is an examle of a media that is supportive and also differential
    BAP
  7. this is an example of a media that is selective and also differential
    MAC
  8. this is the process of taking bacteria from a site and growing it in a culture.
    cultivation
  9. these two bacteria cannot be grown in vitro.
    • leproy
    • syphilis
  10. what three things are needed to sustain microbial metabolism?
    • nutrients
    • atmosphere
    • water
  11. this type of water is recommended to avoid impurities in re-hydrated powder media.
    purified water
  12. this is the original solidifying agent.
    gelatin
  13. this is the most common solidifying agent
    agarose
  14. agarose dissolves at what temperature?
    >95oC
  15. agarose re-solidifies at what temperature?
    <50oC
  16. how much agarose should be used in solid media?
    1-2%
  17. how much agarose should be used in semi-solid media?
    0.1-0.5%
  18. how much agarose should be used in liquid media?
    0.075%
  19. in a THIO broth what is the purpose of agarose?
    • prevents convection
    • prevents penetration of O2
  20. there are partially digested protiens which function as the principal source of organic nitrogen.
    peptones
  21. this is a peptone used in basic media
    trypticase
  22. this is the preferred type of whole blood because it has the correct hemolytic reaction
    sheep's blood
  23. human blood is limited for the use of what organism?
    G. vaginalis
  24. what are the two selective media that horse blood is limited to?
    • R-L media
    • Brucella
  25. why is hemolyzed blood used?
    increases availability of RBC-bound growth
  26. what are the three meat and tissue infusions that are used in media?
    • beef brain
    • heart
    • liver
  27. this provides a carbohydrate additive and a carbon source
    energy substrate
  28. this helps to maintain the isotonic or physiological pH of the cell wall
    sodium chloride
  29. at what percentage is sodium chloide inhibitory
    5%
  30. this helps to maintain H ion concentration.
    buffer system
  31. this is used as an endpoint to ID organisms.
    pH
  32. these help to visually detect pH changes based on bacterial action
    colormetric indicators
  33. what are the colors for phenol red colormetric indicator?
    • acid = yellow
    • alkaline = red
  34. what are the colors for methyl red colormetric indicators?
    • acid = red
    • alkaline = yellow
  35. what are the colors for the neutral red indicator?
    • acid = red
    • alkaline = yellow
  36. what are the colors for the bromcresol purple colormetric indicator?
    • acid = yellow
    • alkaline = purple
  37. what are the colors for the ortho-toluidine blue colormetric indicator?
    • acid = red
    • alkaline = blue
  38. what are the colors for the bromthymol blue colormetric indicator?
    • acid = yellow
    • alkaline = blue
  39. what is the sulfur source for the formation of H2S gas?
    sodium thiosulfate
  40. what is the positive endpoint of an H2S indicator?
    black percipitate
  41. this promote growth of certain groups of organisms
    selective agents
  42. this retards growth of certain groups of organisms
    inhibitory agents
  43. these two terms regarding culture growth are used interchangeably
    • selective agents
    • inhibitory agents
  44. what three dyes are used as GP inhibitors?
    • crystal violet
    • brilliant green
    • basic fuchsin
  45. what is the dye used in the MAC plate?
    crystal violet
  46. what is the dye used in the SS and BS plates?
    brilliant green
  47. what organisms are inhibited by brilliant green?
    • GNC
    • GP
  48. this is used in enteric media to inhibit GNC and GP organisms.
    bile salts
  49. what percent of sodium chloride is inhibitory for all organisms except streptococcus and staphylococcus?
    6.5%
  50. what percent of sodium chloride is inhibitory for all organisms excep staphylococcus?
    7.5%
  51. this inhibits all organisms except salmonella and vibrio
    sodium selenite
  52. what antibiotic is used to inhibit garm positive organisms?
    vancomycin
  53. what two antibiotics are used to inhibit gram negative organism?
    • colistin
    • naladixic acid
  54. what antibiotic is used to inhibit yeast
    nystatin
  55. this media has peptones and is used in susceptibility testing for both fastidious and nonfastidious organisms.
    trypticase soy ager (TSA)
  56. this media has peptones and glucose and is used to speed bacterial growth rate.
    trypticase soy broth (TSB)
  57. This media has beef infusion and starch and used for susceptibility testing and mainttenance.
    mueller hinton ager (MH)
  58. this media has calf brain, beef heart, peptones and glucose, and is used for quality control.
    Brain Heart Infusion Ager (BHIA)
  59. this media is used to accelerate growth to log rate in 6-8 hours. It is the blood culture media base.
    Brain-Heart Infusion Broth (BHIB)
  60. this media contains thioglycolic acid and is used to prevent the formation oand accumulation of toxic H2O2.
    Thioglycollate Medium (THIO)
  61. in THIO pink coloration seen in the uppermost portion of the medium is caused by what?
    Resazurin
  62. what is the O2 indicator in Thioglycollate Medium?
    Resazurin
  63. this is the most frequently used broth in micro.
    THIO
  64. what are three indications for THIO?
    • wound culture
    • culture of small surgical hardware
    • visual evaluation of organism's atmospheric need for O2
  65. this media consists of TSA, brucella, BHI, and is enriched with 5% defibrinated sheeps blood.
    Blood Agar Plate (BAP)
  66. this plate is used in the differentiation of hemolysis
    BAP
  67. this is partial hemolysis, green zone visible due to presence of biliverdin.
    Alpha Hemolysis
  68. this is complete hemolysis, there is a visible clear zone.
    beta hemolysis
  69. this is when there is no hemolysis
    Gamma hemolysis
  70. how are hemolytic reactions enhanced in Blood Ager Media?
    stabbing
  71. streptolysin-O is ______ labile.
    oxygen
  72. this plate has 2% hemoglobin or isovitalex and 5% hemolyzed sheeps RBC's
    Chocolate agar plate
  73. this plate cultivates fastidious organisms needing NAD/Hemin within RBC (Neisseria, Haemophilus).
    Chocolate Agar Plate (CHOC)
  74. this media is selective for N. gonorrhoeae and N. meningitidis.
    Thayer Martin (TM)
  75. this media is more selective than TM when working with mixed flora.
    Modified Thayer Martin (MTM)
  76. what is added to TM plates to inhibit proteus?
    Trimethoprim
  77. this is selective for N. Gonorrhoeae only.
    New York Cityh Agar
  78. the constituents of this plate is peptones, cornstarch, 3% HgB, horse plasma, and antibiotics
    New York City Agar
  79. what three antibiotics are added to Thayer Martin media?
    • Vancomycin
    • Colistin
    • Nystatin
  80. This is a gram positive selective media that has 7.5% NaCl.
    Mannitol Salt Agar (MSA)
  81. what is the pH indicator in MSA?
    phenol red
  82. what media is used as selective for staphylococcus?
    Mannitol Salt Agar
  83. this media is 95% positive for S. aureus.
    MSA
  84. This media is enriched with 5% sheeps blood and selective for GP organisms
    Columbia CNA Agar
  85. this media separates GP organisms from swarming enteric bacteria (e.g. proteus).
    Columbia CNA agar
  86. what is the biochemical agent in MAC?
    lactose
  87. what is the pH indicator in MAC?
    neutral red
  88. what are the two inhibitors in MAC?
    • bile salts
    • crystal violet
  89. on an MAC plate strong lactose fermenters leave a zone of percipitated bile that turns what color?
    pink/red
  90. MacConkey Agar is selective for what organisms?
    GN
  91. this media is enriched with a yeast extract and is used for the recovery of salmonella and shigella from stool samples.
    Hektoen-Enteric Agar (HE)
  92. what are the two pH indicators in HE?
    • bromthymol blue
    • acid fuchsin
  93. what is the H2S indicator in Hektoen-Enteric Agar?
    ferric ammonium citrate
  94. what inhibitor is used in Hektoen-Enteric Agar?
    bile salts
  95. HE is selective for Gram _______ organisms.
    negative
  96. what colors are fermenters on an HE plate?
    yellow/orange
  97. what color do nonfermenters turn on an HE plate?
    • colorless
    • blue
    • blue/green
  98. on an HE plate strong fermenters leave a zone of percipitated bile that is what color?
    orange
  99. what is the pH indicator on the salmonella-shigella Agar?
    neutral red
  100. what biochemical agent do MAC, HE, and SS share?
    lactose
  101. what is the H2S indicator on Salmonella-Shigella Agar?
    Ferric Citrate
  102. what are the three inhibitors on the Salmonella-Shigella Agar?
    • Bile Salts
    • Brilliant Green
    • Sodium Citrate
  103. what is the H2S indicator on Xylose-Lysine-Desoxycholate Agar (XLD)?
    ferric ammonium citrate
  104. this media is used for the isolation of salmonella and shigella from other GNR.
    XLD
  105. what color are fermenters and nonfermenters on XLD?
    • fermenters are yellow
    • nonfermenters are colorless, red
  106. this broth temporarily inhibits normal flora for the isolation of salmonella and shigella.
    GN broth
  107. after 6-8 hours of GN broth sample should be subcultured to what three media?
    • HE
    • SS
    • XLD
  108. gram negative enteric media differentiates what?
    fermenters from non-fermenters
  109. this semi-solid media differentiates by utilizing or not utilizing carbohydrates.
    oxidative-fermentative (O-F) basal medium
  110. What is the pH indicator on O-F?
    bromthymol blue
  111. what are the steps to inoculate O-F tubes?
    • lightly stab 2 tubes 5x
    • add carbohydrate disks aseptically
    • overlay one tube with mineral oil, the other stays open
    • incubate at 35-37oC
  112. in an O-F tube what interpretation is based on the production of what?
    Acid (yellow)
  113. in O-F if acid is produced ony in the open tube the result is:
    oxidative
  114. in O-F if acid is produced in both tubes the result is:
    fermentative
  115. in O-F if acid is produced in neither tube the result is:
    non-saccharolytic
  116. this is a semi-solid medium with a cystine biochemical that is used to differentiate neisseria species.
    cystine-trypticase agar (CTA)
  117. what is the pH indicator in CTA?
    phenol red
  118. this agar has a biochemical of deoxyribonucleic acid at 0.2%.
    Dnase Agar
  119. what is the indicator in Dnase agar?
    ortho-toluidine blue
  120. the purpose of this media is to detect bacterial DNase production
    DNase agar
  121. Dnase agar is ___ positive for S. aureus.
    99%
  122. DNase is ___ positive for S. marcescens.
    97%
  123. what is the appearance of a positive result on DNase?
    rose pink
  124. what is the media protocol for throat cultures?
    BAP
  125. what is the media protocol for nasopharyngeal cultures?
    • BAP
    • CHOC
  126. what is the media protocol for sputum?
    • CHOC
    • BAP
    • MAC
  127. what is the media protocol for urine?
    • BAP
    • MAC
  128. what is the media protocol for wound cultures?
    • CHOC
    • BAP
    • MAC
    • THIO
  129. what is the media protocol for stool?
    • BAP
    • MAC
    • HE
    • CAMPY
    • GN
  130. what is the media protocol for genital cultures?
    • CHOC
    • BAP
    • MAC
    • MTM
  131. what is the media protocol for CSF?
    • CHOC
    • BLOOD CULTURE BOTTLE/THIO
  132. what size glassware is used to prepare media?
    twice as large
  133. unprepared media is extremely __________.
    hygroscopic
  134. small loads should be sterilized at what temp? for how long?
    • 121oC
    • 15 min
  135. large loads should be sterilized at what temp? for how long?
    • 121oC
    • 20-30 min
  136. at what temperature do you add blood to the mdium for CHOC?
    75-80oC
  137. at what temperature do you add enrichments including whole blood?
    45-50oC
  138. at what temperature should the medium be poured into a petri dish?
    50-55oC
  139. how do you eliminate bubbles in a medium?
    pass flame on surface
  140. how do you eliminate condensation on a petri dish?
    lid ajar for 15-20 min
  141. how much medium should be used to achieve a 3mm depth in a 100 mm petri dish?
    20mL
  142. how much medium should be used to achieve a 5-6mm depth in a 150mm petri dish?
  143. what is the shelf life of media?
    2 weeks to four mounths
  144. what type f media has a longer shelf life?
    tubed
  145. what percent of prepared plates should be tested for quality control?
    5%
  146. what is the ideal temperature for incuabtion?
    35-37oC

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