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Author:
DesLee26
ID:
296957
Filename:
Article
Updated:
2015-02-26 16:49:17
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Sam
Folders:
MolecularTestTwo
Description:
Test two
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  1. degron
    a domain that signals ubiquitylation of proteins

    in some, it overlaps with a transcriptional activation domain
  2. Ub-ligases
    tag substrates by interacting with the degron and recruiting Ub-conjugating enzymes to the substrate protein
  3. SCF
    Skp, Cullin, F-box containing complex (or SCF complex) is a multi-protein E3 ubiquitin ligase complex catalyzing the ubiquitination of proteins destined for proteasomal degradation
  4. GAL1
    promoter that is involved in galactose catabolism

    in the experiment, it was modified with two LexA binding sites to drive expression of Beta galactosidase
  5. ChIP analysis
    • used to analyze protein interactions with DNA
    • combines chromatin immunoprecipitation with DNA sequencing to identify the binding sites of DNA associated proteins
  6. ARS1 region
    early-firing autonomously replicating sequence
  7. pulse-chase analysi
    method for examining cellular processes as they occur over time by successively exposing the cells to a labeled compound (pulse) and then to the same compound in an unlabeled form (chase).
  8. pull down assay
    in vitro method used to determine a physical interaction between two or more proteins; useful for both confirming the existence of a protein-protein interaction predicted by other research techniques and as an initial screening assay for identifying previously unknown protein-protein interactions.

    affinity chromatography is used; used to pull proteins out of solution

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