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a domain that signals ubiquitylation of proteins
in some, it overlaps with a transcriptional activation domain
tag substrates by interacting with the degron and recruiting Ub-conjugating enzymes to the substrate protein
Skp, Cullin, F-box containing complex (or SCF complex) is a multi-protein E3 ubiquitin ligase complex catalyzing the ubiquitination of proteins destined for proteasomal degradation
promoter that is involved in galactose catabolism
in the experiment, it was modified with two LexA binding sites to drive expression of Beta galactosidase
- used to analyze protein interactions with DNA
- combines chromatin immunoprecipitation with DNA sequencing to identify the binding sites of DNA associated proteins
early-firing autonomously replicating sequence
method for examining cellular processes as they occur over time by successively exposing the cells to a labeled compound (pulse) and then to the same compound in an unlabeled form (chase).
pull down assay
in vitro method used to determine a physical interaction between two or more proteins; useful for both confirming the existence of a protein-protein interaction predicted by other research techniques and as an initial screening assay for identifying previously unknown protein-protein interactions.
affinity chromatography is used; used to pull proteins out of solution
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