immuno 008 antibodies part 2 (C-regions V-regions H-chain L-chain immunoglobulin domains epitop

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Author:
mikepl103
ID:
297129
Filename:
immuno 008 antibodies part 2 (C-regions V-regions H-chain L-chain immunoglobulin domains epitop
Updated:
2015-03-05 22:30:14
Tags:
immuno 008 antibodies part regions chain immunoglobulin domains epitope IgM IgG IgA IgE IgD 14
Folders:
2015,biology,immunology
Description:
immuno 008 antibodies part 2 (C-regions, V-regions, H-chain, L-chain, immunoglobulin domains, epitope, IgM, IgG, IgA, IgE, IgD) #14
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  1. the antigen-binding sites of antibodies are usually rich in what types of amino acids?
    aromatic amino acids (Phe, Tyr, Trp) which can form strong hydrophobic interactions
  2. what are the main characteristics of IgM antibodies?
    it is the first antibody produced in response to a pathogen. By isotype switching, synthesis of IgM gives way to synthesis of IgG
  3. what are the main characteristics of IgG antibodies?
    it's the most abundant antibody. IgG is smaller and more flexible than IgM. During pregnancy, it can be transferred across the placenta to provide the fetus with protective antibodies from the mother
  4. what is remicade?
    a monoclonal antibody neutralizing TNF; used in rheumatoid arthritis.
  5. Herceptin
    binds protein (HER2) expressed on some tumor cells (breast cancers, lymphomas); inhibits cancer cell proliferation
  6. what are four immunological methods based on antibodies?
    ELISA, Western Blotting, Immunofluorescence, immunoprecipitation
  7. explain how Western Blotting works
    • 1. proteins separated by electrophoresis on a protein SDS-PAGE.
    • 2. proteins transferred to (nitrocellulose or PVDF) membrane sheets.
    • 3. protein bands visualized with enzyme tagged antibodies

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