mb 230 4-3

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  1. 2 forms of genetic recombination
    • 1) vertical gene transfer
    • 2) horizontal gene transfer
  2. vertical gene transfer is done through
    • 1) sexual and asexual reproduction
    • 2) parent to offspring
  3. Horizontal gene transfer is done through
    • 1)asexual transfer
    • 2) parent to parent
  4. Parent to offspring:
    1) Type of gene transfer
    2) what is the recombination gene transfer
    • vertical gene transfer:
    • recombination of parental genes (traits)
  5. what is the recombination of parent to parent genes in gene transfer
    • Horizontal gene transfer resulting in:
    • 1) recombination of shared genes
    • 2) addition of shared genes to current genetic material
  6. 3 methods for genes transfer in bacteria
    • 1) conjugation
    • 2) transformation
    • 3) Transduction
  7. Define conjugation
    direct transfer of DNA from one bacterium to another involving the F plasmid.
  8. What is the F plasmid and what does it do?
    • The fertility (F+) plasmid contains:
    • 1) genes for translating/transcribingthe sex pilus
    • 2) genes to transfer replicated F plasmid through sex pilus
  9. What are F+ bacteria
    contain the plasmid that can initiate conjugation
  10. what is F- bacteria
    Cannot initiate conjugation, and do not contain F plasmid prior to conjugation
  11. what results from conjugation
    • -The F- becomes F+, because F+ inserted a copy of the plasmid & genes for sex pilus.
    • -If F+ has resistance to antibiotics, so will F- after conjugation.
  12. Define Transformation
    -Horizontal gene transfer of 'naked' DNA, which is picked up from the environment by bacterium.
  13. where does naked DNA come from?
    a cell that died or was destroyed (donor cell).
  14. What do competence factors do?
    enable DNA to enter bacterial cell during transformation in horizontal gene transfer.
  15. What is transduction
    Horizontal gene transfer of DNA from one bacterium to another by a virus (bacteriophage).
  16. 4 steps in genetic engineering
    • 1) isolate DNA interest Gene Of Interest (GOI)
    • 2) Cut DNA w/restriction enzymes
    • 3) Combine DNA pieces together
    • 4) Transform cell with G.E. DNA
  17. The ___ DNA acts as a taxi cab for ___
    Vector DNA, GOI
  18. How do we cut DNA w/restriction enzymes
    restriction endonucleases
  19. How do we combine DNA pieces together
    GOI+Vector DNA+ Ligase=G.E. DNA
  20. How does RE decide where to cut
    Restriction Endonucleases recognize a sequence of DNA, and cut between two amino acids.
  21. What enzyme is added to the sticky ends of the GOI to the vector DNA?
    DNA Ligase
  22. In order to make protein(s) from your GOIn what do you need to do with your new GE DNA?
    Transformation needs to occur. Transcription and translation within cell transforms proteins.
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mb 230 4-3
2015-03-13 04:35:01
mb 230
mb 230 4-3
mb 230 4-3
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