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what is the purpose of staining blood smears?
so RBC , WBC and platelets can be microscopically viewed.
What specimen can be used to prepare blood smears?
- Capillary blood with no anticoagulant
- Venous blood with EDTA w/in 2 hours of blood collection.
what blood components can be viewed on stained smear?
what are 5 errors to avoid when making a blood smear?
- Smear is:
- too thin or too long
- too thick or too short
- Ridges waves in smear
- Uneven cell distribution
- Artifacts or unusual cell appearance
Name two commonly used blood stains?
How should a properly stained smear appear?
- pinkish-blue to the naked eye
- microscopically: RBC: pink-tan
- nucleus or central structure: purple
- Cytoplasm: very from pink to blue, or blue-gray
- depending on the cell type.
Name the three factors that can affect staining results?
- pH variation
- characteristic of stain or buffer
Smears that are too pink can be caused by?
- stain time: too short
- wash time: too long
- pH of stain or buffer: too acidic
Smears that are too blue can be caused by?
- over staining
- wash or buffer time: too short
- pH of the stain or buffer: to alkaline
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