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Why do we use embryo transfer?
prevent interruption of mare's performance career
get more than one offspring from a mare each year
obtain foals from mares that aren't able to maintain pregnancy
marketing of embryos
What kinds of embryo transfer are there:
disadvantages of ET:
need a well trained technician
usually can only get one embryo at a time
sub-fertile mares are usually less productive
aged mares have embryos that has less survivability for ET
What makes ET successful?
management of recept
What is regimate?
a progesterone supplement
What stimulates ovulation?
What needs to be given to recept mare?
progesterone until accessory CLs develop or the fetus is producing enough to maintain pregnancy
characteristics for selecting a recept:
similar in size or larger than donor
age 3-10 ideal
sound repro history
good maternal characteristics
1. synchronize ovulation
2. breed donor mare (day of ovulation=day 0)
3. flush uterus between day 6-8
4. wash embryo and load into transfer instrument
5. place embryo in uterus of recept mare
for Estrus Synch:
leutalise is a common prostaglandin
usually given twice, 14 days apart
must have a CL
10 day or 14 day schematic
at end of schematic give Leutalise
given to mares without ovaries
helps get recept and donor to similar follicular development and ovulation
What can you do in order to skip the synching process?
using an Ovect mare progesterone about 2 days after the donor mare but you have to keep giving recept mare progesterone until day 150
How do you prep the donor for ET?
day 7 after ovulation
clean out rectum
remove from feed or use rectal suppressant
Embryo collection procedure:
use Foley Catheter
flush mare with 1 liter of collection media
siphon each liter out through an embryo collection filter
repeat flushing 3-4 times
search contents of filter
How do you flush the filter?
rinse with serum and search with a microscope
do this as quickly as possible
How are embryos graded?
according to viability
uniform blasphomeres and not disintegrating
Grade 5 is worst
How do you mange a recept mare after ET?
keep on a regimate supplement
check to see if keeping pregnancy
check for growth
How are embryos transported to mares in other places?
similar to semen
keep in media instead of extender
embryo placed in tube instead of syringe or bag
cooled in equitainer
avg viability is 24 hours