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an immature erythrocyte that still contains RNA remnants in the cytoplasm is?
Reticulocyte
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A decrease below the normal number of reticulocytes in the circulating blood
Reticulocytopenia
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an increase above normal number of reticulocytes in the circulating blood is?
reticulocytosis
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What are two dyes (stains) used to stain reticulocytes?
- new methylene blue
- brilliant cresyl blue
- both isotonic solution
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Why would a reticulocyte count be ordered?
- to determine the cause of low RBC count or anemia
- to classify the type of anemia
- monitor the effectiveness of anemia treatment
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T or F: the reticulocyte count estimates the number of immature RBC in the circulating blood. therefor it is an indirect way of estimation erythropoiesis ( RBC production)?
TRUE
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How is a standard reticulocyte count performed?
- Dried smear is examined microscopically with oil immersion objective
- total of 1000 RBC are counted
- Number of reticulocytes observed per 1000 RBC is recorded
- Reticulocytes are counted twice- once as retic, once as red cell.
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How is the Miller reticle used to perform a reticulocyte count?
- The Miller reticle imposes two squares—a large square and a smaller inner square
- For each field of view, the number of reticulocytesis counted in the entire large square (including the small square)and recorded;
- all red cells lying within the small square arecounted, including reticulocytes, and that number is recorded asred cells.
- This is repeated for several fields until 100 to 200 redcells have been counted in the small squares and recorded.
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What is the normal reticulocyte reference range for adults? For newborns?
- adult: 0.5-1.5%
- newb: 2.5-6.5%
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What conditions can cause reticulocytosis?
- Hemolytic anemia
- Hemorrhage
- Living a high altitudes
- Heavy smoking
- Factor deficiencies (folate, iron, B12)
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What conditions can cause reticulocytopenia?
- Aplastic anemia
- Vitamin B12 deficiency
- Iron deficiency anemia
- Bone marrow failure
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State the formula for calculating a standard reticulocyte count.
% Retics = (# Reticulocytes counted / # RBC counted ) x 100
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Formula for the absolute reticulocyte count
- Absolute reticulocyte count =
- RBC count x % Reticulocytes
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Formula for corrected reticulocyte count ?
Correctedretic count =
Retic count(%) x ( Patient Hct/45)
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nontoxic dye used to stain living cells or tissues
supravital stain
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