The flashcards below were created by user
caitlin
on FreezingBlue Flashcards.
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Resolution
the ability to distinguish closely positioned objects as separate images
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Resolving power of human eye
0.2 mm
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resolving power of LM
0.2 microm
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resolving power of TEM
1 nm
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Resolving power of SEM
2.5 nm
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General Histological Approach
- 1. Fix
- 2. Infiltrate and embed
- 3. Stain
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Acid Dye
Organic or inorganic compound that carries a permanent negative charge
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Basic Dye
Organic or Inorganic compound with a permanent positive charge
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Enzyme Histochemistry
the location of an enzyme is determined by taking advantage of its catalytic activity
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Schiff Reagent
PAS method to localize carbohydrates
Fuelgen reaction to stain DNA
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Immunocytochemistry
antibodies used to locate specific molecules (antigen)
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Autoradiography
localization of radiolabeled molecules
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In Situ Hybridization
used to detect RNA or DNA using nucleic acid probes to locate complementary nucleotide sequences
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