What was the study done to test whether single stem cells are responsible for all blood cells?
They exposed an animal to high doses of x-rays, destroying most of the hematoopoietic cells, causing the animal to die within a few days. If, however, the animal receives a bone marrow transplant, it allows recovery and repopulation of bone marrow.
Cells are then sorted and the different fractions are transfused back into irradiated mice. If a fraction rescues an irradiated host mouse, it must contain hemapoietic stem cells. Cells are then labeled to figure out stem cells
If a fraction rescues an iradiated host mouse, it has hemopoietic stem cells.