Lecture 11: Microbial Growth (Quiz 6)

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Lecture 11: Microbial Growth (Quiz 6)
2015-05-15 21:13:56
how bacteria grows
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  1. Microbial Growth
    • By numbers, not by size
    • More specifics on cell division than just divide down the middle
  2. FtsZ
    • Main Protein in bacterial cell division
    • 1 ATP created per FtsZ
    • A Z-Ring is formed, it recruits about 20 cell division proteins and squeezes the cell in half (finishes the division).
    • Inhibited by MinCDE, SulA, and SlmA
  3. MinCDE
    • Prevents z-ring on the poles
    • Oscilate back and forth, this is how the cell knows that it is getting longer and when to divide
    • 3 different proteins
    • MinD: Completely coats a quarter of the cellĀ 
    • MinC: Connects to to D, does the same job as SlmA
    • MinE: Forms ring, moves towards D and C
  4. SlmA
    • Prevents z-ring over DNA
    • Binds everywhere there is DNA
    • Gets in between monomers so Z's can't connect
    • Stopping cell division
  5. SulA
    • Prevents z-ring when DNA is damaged
    • There to detect DNA damage
  6. Senescence
    • Cells growing older
    • Prokaryotes get older by what their cell wall looks likes
    • The older the cell, the weaker the cell wall
  7. Bacterial Growth Curve
    • Lag Phase: Adjustment, growing in size
    • Log Phase: Exponential, rapid cell division, when antibiotics work best
    • Stationary Phase: 1 born, 1 dies, nutrients running out, horizontal gene transfer, spore making
    • Death Phase: No nutrients
  8. Nonsynchronous Growth
    • Cell division
    • Cells aren't dividing at the same time due to the age of the cell
  9. Synchronous Growth
    • Cell Division
    • All of the cells are growing at the same time
  10. Double Times
    • How fast bacteria split into 2
    • E. Coli is very fast
    • 20 min doubling time
  11. Bacterial Growth Temperature
    • Psychrophiles: VERY cold (the coldest), modified fatty acids (unsaturated)
    • Psychotrophs: Moderately cold
    • Mesophiles: 37 degrees C, Humans
    • Thermophiles: Hotter
    • Hyperthermophiles: VERY hot (the hottest), heat love orgs., High G/C ratio, tight knit proteins
  12. Food Micro
    • Storing food in smaller containers after cooking helps it cool faster and prevents food from going into the danger zone of temperature, where there is rapid growth of bacteria.
    • Bacillus Cereus: The bacteria associated with rice
  13. Acidophiles
    • They love acid!
    • pH 0.1 to 5.4
    • Modified Protein Pumps
    • Snottites: Bacteria that hangs from cave walls that have a very high acidity content and have the consistency of snot