Micro Practicum Slides

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Micro Practicum Slides
2015-05-27 20:37:10
Micro Practicum Slides

Micro Practicum Slides
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  1. ID the bacterial organelle shown, Give 1 function, give the gram reaction
    • ID: Capsule
    • Function: biofilm formation or attachment,
    • Gram: unknown undetermined (cuz capsule stain not gram stain)

  2. ID the plant pathogen shown (genous
    and species), comment on clinical significance (give 1 )
    • ID: Claviceps purpurea
    • Significance: Ergotism (produces potent halucigen). or st. vit’s fire
    • Suggested as a treatment for migrane, useful for septic abortion

  3. This is a liver biopsy of a marine recently returned from afganistan. ID the org (genous and species), diagnose the disease, name the vector
    • ID: Leishmania donovanii
    • Disease: Dum dum feaver or leishamaniasis
    • Vector: brown sand fly

  4. Week long history of cheesy foul smelling discharge from genetalia a monogomous female. ID org, diagnose disease, name the stage, how was this likely acquired
    • ID: Trichomonas Vaginalis.
    • Disease: Vaginitis.
    • Stage: trophozoite (because of flagella).
    • Transmitted: STD

  5. Blood smear from a native from sudan with a 3 week history of recurring fevers. Id org. Diagnose disease. Name the vector.
    • ID: Plasmodium
    • Disease: Malaria
    • Vector: Female anoepheles mosquito

  6. ID the parasitic flat worm shown. Give scientific name of species. Give the
    common name. Where would you expect to see this org?
    • Scientific Name: Clonorchis
    • Common Name: Siniensis
    • Origin: Asian liver flue. Asia

  7. Name the structure shown. ID the org (genous or species) from which this was taken. How is this typically acquired?
    • Structure: Scolex
    • ID: Taenia Solium also known as the pork
    • tape worm.
    • Acquired: eating contamined meat

  8. ID the colonial algae. Comment on Significance
    • ID: Volvox
    • Significance: Photosynthetic so it produces oxygen and is the basis of aquatic food chains

  9. Name common fungus shown. Give stage. Clinical Significance?
    • ID: Rhizopus
    • Stage: Zygospore
    • Significance: Opportunistic mycoses in compromised host

  10. Name common environmental fungus shown. What is the clinical Significance?
    • ID: Aspergillus
    • Significance: Produces aflatoxin, can also cause ling and ear infections and disseminates compromised host

  11. This yeast is normal flora in human guy and genetalia. ID org. Significance?
    • ID: Candida Albicans
    • Significance: Fungal vaginitis and is also the leading cause of death neutropenics

  12. Name the fungus shown. Significance.
    • ID: Penicillium
    • Significance: Produces antibiotic penicilin and causes alergic responses

  13. Name the parasitic flat worm shown. Common name? How is it acquired?
    • ID: Fasciola Hepatica
    • Common Name: Sheep Liver Fluke
    • Acquired: Eating contaminated wild game

  14. This is a blood smear from a native in africa. Id the org. Dx disease. Vector
    • ID: Trypanasoma
    • Disease: African Sleeping sickness
    • Vector: Te-Tse Fly

  15. This is a urethral smear from a 25 year old premiscuous male who thinks that only wimps wear condoms. Gram - diplococci are shown. ID, Disease, Acquired?
    • ID: Nisseria Ghonorriha
    • Disease: Ghonorriaha or the Clap
    • Acquired: STD

  16. Name the organelle shown. Function? Suggest a likely ID of org based on characteristic morphology
    • ID: Flagella
    • Function: Locomotion
    • Likely Org: Proteus species since it has swarmer cells

  17. This is a fecal specimen that is taken from a peace core worker recently returned from panama with a week long history of bloody watery diarrhea. ID, Disease, Stage, Acquired?
    • ID: Entamoeba Histolytica
    • Disease: Amoebic Dysentery
    • Stage: Trophozoite (cuz 1 nucleus)
    • Acquired: Drinking fecally contaminated water

  18. This is a muscle biopsy of a foot ball player who was fed smoked mountain lion by his coach. ID, Disease, Acquired?
    • ID: Trichinella Spiralis
    • Disease: Trichinosis
    • Acquired: Eating contaminated meat

  19. Austins name? This is a trichrome stain from a 2 year old child who is not potty trained and has watery diarhea. ID, Disease, Stage, Acquired
    • ID: Giardia Lamblia
    • Disease: Giardiasis
    • Stage: Trophozoite
    • Acquired: Drinking fecally contaminated water

  20. Name the bacteria organelle shown(blue). Likely ID, Gram reaction, How do you know this?
    • Organelle: Endospore
    • ID: Bacillus
    • Gram: Gram +
    • How?: Cuz only gram + bacteria form spores

  21. Give gram reaction. Structure. ID
    • Gram: Gram +
    • Structure: Endospore
    • ID: Clostridium

  22. Arm of a truck driver 3 days after accident. A gram + obligate anaerobic spore forming material was isolated from necrotic tissue with cellulitis. What is diagnosis? ID
    • Diagnosis: Gas Gangrene
    • ID: Clostridium Perfringenes

  23. 2 part slide: Hair dresser 3 days after surgery to fix a broken hip. A gram +, coagulase +, catalase + coccus was isolated from his blood 3 days after surgery. ID, Disease process, Why?
    • ID: Stapholoccocus Aerueus
    • Disease: Post operative toxic shock syndrome
    • Why?: Unusual because the is seen in females

  24. 2 part slide: Hair dresser 3 days after surgery to fix a broken hip. A gram +, coagulase +, catalase + coccus was isolated from his blood 3 days after surgery. ID, Disease process, Why?
    • ID: Stapholoccocus Aerueus
    • Disease: Post operative toxic shock syndrome
    • Why?: Unusual because the is seen in females

  25. Fecal specimen from patient. ID, Stage, Disease, Acquired?
    • ID: Giardia Lambia
    • Stage: Trophozoite
    • Disease: Giardiasis
    • Acquired: Drinking contaminated fecal water

  26. Name the pattern of hemolysis shown. Give 1 org that produces this
    • Patter: Complete or Beta Hemolysis
    • Organism: Stapholoccocus Aeurueus
    • Urea Hydrolysis
    • Pink: Positive
    • Org: Proteus
    • Citrate Test
    • Blue = positive
    • Enterobacter Aerogenes
    • Gelatin Test:
    • Above = positive
    • Org: Clostridium Perfringens
    • Casein Hydrolysis Test
    • Above is positive
    • Org: Bacillus cereus
    • Starch Hydrolysis
    • Below is positive
    • Org:Bacillus cereus
    • Oxidation-Fermentation Test
    • Yellow: Oxidation and Fermentation
    • org: Escherichia
    • Manitol Salt Agar
    • Top: fermented mannitol
    • Org: Staphylococcus aureus, yellow with halo
    • MacConkey Agar
    • Pink = lactose fermenters
    • Org: Escherichia Coli
    • Eosin Methylene Blue Agar (EMB)
    • Top is lactose fermenter
    • Org: Escherichia coli
    • Nitrate Test
    • Nitrate reduction: Red after reagents
    • Org: Escherichia coli
    • Amino Acid Decarboxylation Test
    • Lysine positive is on left and lysine negative is on the right 
    • Org: Proteus Vulgaris
    • TSIA
    • red slant = K
    • Yellow butt = A
    • no change = NC
    • Escherichia coli
    • Phenol Red/Carbohydrate Fermentation
    • Lactose and glucose + for E.Coli cuz acid gas production
    • Sucrose - because red and no bubble
    • Fluid Thioglycollate Medium (FTM)
    • E.coli positive for growth in aerotolerance
  27. Microscope Formula
    • X*10/Y
    • X = Stage
    • Y = Oculars