Phlebotomy (Day 1)
Card Set Information
Phlebotomy (Day 1)
What is the function of Public Health services?
To screen, monitor, and educate about infectious diseases
DO NOT provide treatment
STudy of nonverbal communication (body language)
Who developed the
Guideline for Isolation Precautions in Hospitals
(center for disease control) and
(healthcare infection control practices advisory committee)
*NOTE - OSHA BBP standard requires gloves during phlebo
Requirement to provide MSDSs
Layers of skin + what's found there
: layer of epidermis
: layer of epidermis
: blood vessels
: blood vessels, adipose tissue
Layers of blood vessel (both names) + brief description
Tunica adventita (externa)
: outer layer
made of CT
thicker in arteries
: middle layer
smooth muscle + elasic fibers
much thicker in arteries
Tunica intima (interna)
: inner layer
single layer of endothelial cells + basement membrane, CT layer, and elastic membrane
Which are the anticubital veins?
Acessory cephalic, median basilic, median
*NOTE- subclavian is shoulder -> chest
What are these things - CK, LD, ALP, ALT, BUN, PT, GTT, ESR
: creatine kinase - enzyme in cardiac muscle
: lactate dehydrogenase - enzyme in cardiac muscle
(both released during myocardial infarction)
: alkaline phosphatase - enzyme that determines liver function
: alanine aminotranfersale - enzyme that determines liver function
: blood urea nitrogen - kidney function test
: glucose tolerance test - glucose metabolism test
: erythrocyte sedimentation rate - nonspecific disease indicator (esp inflammatory conditions)
NPO (nil per os): nothing by mouth (not even water)
Describe the methods of anticoagulation and their agents
binding/chelating or ppt calcium to make it unavailable for the coag process
: Sodium citrate, oxalate, EDTA, SPS
inhibiting formation of thrombin
: lithium and sodium heparin
Most common antiglycolytic agents? Purpose?
sodium fluoride and lithium iodoacetate prevent glycolysis (metabolism of glucose)
When should a discard tube be used?
When drawing for a coag test (not PT or PTT) because tissue thromboplastin activates extrinsic coag pathway
what is accessioning the specimen?
Steps taken to unmistakably connect a specimen and the accompanying paperwork to a specific individual
What veins should you use? Which should you avoid?
Use - cephalic or median cephalic
Don't use - basilic (too close to brachial artery)
Tourniquet should not be left on for more than...
Release after blood flows into first tube
What fluids are contained in a capillary specimen?
tissue fluids (interstitial and intracellular)
What reference values shift with capillary draws?
: Calcium, Phosphorous, and total protein
What does an ABG req typically include?
method of ventilation/delivery
normal patient ID info
What is the modified Allen test?
compression of both the ulnar and radial arteries to stop arterial flow to the hand
hand should appear blanched
IF collateral circulation is present hand will flush pink when only ulnar artery is released (positive result)
Name 4 analytes that need LP
Name 3 tests that require cooling.
name a tests that can be damaged/altered by cooling
: cold inhibits glycolysis which causes accumulation of K outside the cell (elevated results)
Cold also causes hemolysis which elevates results
How quickly must an ESR be performed on an EDTA spec at room temp? When refriged?
: 4 hours
: 12 hours
Maximum time limit for serum/plasma separation?
2 hours from collection (should be ASAP)
What departments issue regulations re: off-site transportation of spec?
DOT (department of transportation)
FAA (federal Aviation administration)
OSHA (occupational safety and health administration)
What is unique about an ammonia spec?
must be transported ASAP and on ice
serum vs plasma
: does not contain fibrinogen (used during clotting)
: contains fibrinogen (no clotting)
What is a C&S? What is it's purpose?
Culture and sensitivity
diagnosis of UTI
what is AFP?
: antigen found in fetus, amniotic fluid, and maternal serum
abnormal values can indicate developmental issues
What is POCT?
Point-of-care testing (performed at/near site of patient care)
Interpretations of a TB skin test
based on induration (firm, raised area)
What do POCT urine test strips usually detect?
gluocse, bilirubin, bacteria, blood, pH, protein, specific gravity, urobilinogen
What organizations offer certification to lab professionals?
AMT (american medical technologists)
ACA (american certification agency)
ASCP (american society for clinical pathology)
what organizations accredit phlebotomy programs?
NAACLS (national accrediting agency for clinical laboratory services)
PHS (public health services)
what is PHI?
Protected health information
What is CLIA '88?
clinical laboratory improvement amendments of 1988
established standards for regulation of laboratories
CLSI standards state that heel puncture depth should not exceed ____
What is CLSI?
Clinical & Laboratory Standards Institute
publishes standards for phlebotomy procedures
What are diurnal variations?
normal fluxuations that occur throughout the day (think diurnal cycle)
Hematomas - where can you collect? Why?
Distal to, or below (blood flow least affected)
Collecting in the area of a hematoma can cause pain and yield erroneous results