Biochemical Methods for studying Proteins

Card Set Information

Author:
mnvang321
ID:
305170
Filename:
Biochemical Methods for studying Proteins
Updated:
2015-07-13 23:57:28
Tags:
biochem
Folders:
biochem
Description:
exam 1
Show Answers:

Home > Flashcards > Print Preview

The flashcards below were created by user mnvang321 on FreezingBlue Flashcards. What would you like to do?


  1. separate proteins based on their binding to specific chemicals
    Affinity chromatography
  2. separate proteins based on pI
    Isoelectric focusing
  3. separate proteins based on mass
    Gel-filtration chromatography
  4. separate proteins based on charge
    Ion-exchange chromatography
  5. reduces protein's solubility in water
    Ammonium sulfate
  6. separate protein based on mass
    SDS-PAGE
  7. detects specific proteins thru specific binding to antibodies only
    ELISA
  8. separate proteins based on their binding characteristics
    Affinity chromatography
  9. SDS-PAGE stands for...?
    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
  10. ELISA stands for...?
    Enzyme Linked Immuno Sorbent Assay
  11. A protein sequencing method that requires the chemical Phenylisothiocyanate to react with the alpha amino group on AAs to produce a phenylthiohydantoin-derivative of the AA.
    Edman degradation
  12. utilizes both electrophoresis and immunological techniques to detect a protein
    Western Blot
  13. Carboxyl side of methionine residues
    Cyanogen bromide
  14. Carboxyl side of lysine and arginine residues
    Trypsin
  15. Carboxyl side of tyrosine, tryptophan, phenylalanine, leucine, and methionine.
    Chymotrypsin

What would you like to do?

Home > Flashcards > Print Preview