Ch3

Card Set Information

Author:
BodeS
ID:
307595
Filename:
Ch3
Updated:
2015-09-11 21:05:35
Tags:
microbiology
Folders:

Description:
microscope
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  1. eye piece lens
    remagnifies the i mage formed by the objective lens
  2. objective lenses
    primary lenses that magnify the specimen
  3. stage
    hold the microscope slide in position
  4. stage manipulator
    2 knobs that moves the stage right, left, backwards and forwards.
  5. condensor
    focuses light through specimen
  6. fine focus
    final adjustments image of specimen
  7. course focus
    initial move of stage all the way up during 10X objective
  8. total magnification
    • 4X
    • 10X
    • 40X
    • 100X
  9. resolution
    • ability of lenses
    • to distinguish
    • fine detail and structure
  10. darkfield microscopy
    fxn
    special feature
    • fxn: external structural detail
    • when staining is not ideal 
    • special feature: darkfield condensor
    • contains opaque disk
    • only reflected off light hits specimen
  11. phase-contrast microscopy
    fxn
    special feature
    • fxn: internal structural detail
    • special feature: reinforcement (bright)
    • interference (dark)
    • diffraction (reflected off)
    • light rays on different phases from different sources interact
  12. brightfield microscopy
    ID
    fxn
    special feature
    • ID: compound light microscope
    • fxn: stains
    • special feature: by focusing the light, the condenser produces a brightfield illumination
  13. electron microscopy
    fxn
    fxn: used to see viruses
  14. stain
    salts containing positive and negative ions
  15. staining
    color specimen with dye to emphasize structural presence
  16. fixed
    • attach microorganism to slide
    • ex: heat-fixed
  17. smear
    film of specimen spreads over slide upon fixing
  18. basic dyes
    • chromophores
    • in stain
    • that come from 
    • positive ion
  19. acidic dyes
    • chromophore
    • in stain 
    • that comes from
    • negative ions
  20. negative staining
    • fxn: capsule staining
    • pathogen: Klebsiella pneumoniae
  21. simple stain
    alcohol solution of basic dye
  22. mordant
    iodine
  23. differentail stains
    • Gram stain 
    • acid-fast stain
  24. Gram stain
    • classifies bacteria into two large groups 
    • Gram positive/negative
  25. primary stain
    crystal violet
  26. decolorizing agent
    alcohol
  27. Gram-positive
    purple blue dyed bacteria
  28. Gram-negative
    red pink dyed bacteria
  29. counterstains
    • safranin 
    • contrasting color to primary stain
  30. acid-fast stain
    • genus: Mycobacterium
    • pathogen: Mycobacterium tuberculosis
  31. specialized stains
    • Endospores
    • ex: Bacillus cerus
    • Flagella
    • ex: Bacterial flagella
    • Negative
    • ex: capsule staining
  32. immersion oil
    • fxn: preserve the direction of light rays
    • at 100x
  33. stains
    simple 
    differential 
    structural/specialized

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