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Every blood draw is __
What animal can get a vacutainer blood draw on a jugular vein?
What animal can get a vacutainer blood draw on a cephalic or femoral?
cats and dogs
What animal can get a vacutainer blood draw on a saphenous?
How do you select sight choice?
- health of animal
- quantity of blood needed
What is a vacutainer?
a tube containing a pre measured vacuum that will draw a specific amount of blood into a tube
What should you do before inserting a needle when using a vacutainer? (2)
- know what kind of sample you are getting
- gather supplies (plus extras!)
what does the needle attach to when using a vacutainer?
what are the three pieces used when drawing blood with a vacutainer?
- needle (single or multi use)
Steps to drawing blood with a vacutainer:
- 1) attach sterile needle
- 2) Slide in vacutainer (not all the way, leave vacuum)
- 3) insert needle
- 4) push on vacutainer activating vacuum
- 5) when completely finished pull back until vacuum stops and pull out needle
- 6) apply pressure
What two ways can vacutainers be filled?
by hand or needle
Problems with Vacutainers
- Expired tubes
- back flow into patient
- micro clots
- sterility is compromised if tube is not disengaged prior to removal from patient
When do you use a vacutainer?
- What type of blood needs to be drawn
- multiple samples from one poke
When getting a whole blood tests from a dog or cat why use a vacutainer?
because their blood clots quickly
What are some human errors when using vacutainers?
- have to wait to clot
What is a special about a yellow vacutainer? What is it used for?
- it is sterile, no additives
- used for cultures
What is a special about a red vacutainer? What is it used for?
- no additives
- spun to obtain serum
What is a special about a lavender vacutainer? What is it used for?
- EDTA (inhibits clotting) not detectable
- used for a CBC
What is a special about a green vacutainer? What is it used for?
- has heparin (type of clotting but detectable)
- peripheral blood/ bone marrow
What is a special about a blue vacutainer? What is it used for?
- sodium citrate
- coagulation essays
What is a special about a black vacutainer? What is it used for?
What is a special about a grey vacutainer? What is it used for?
- sodium floride
- glucose, anticoagulant
What is a special about a brown vacutainer? what is it used for?
lead in organ
what is special about a grey/red vacutainer? What is it used for?
- mottled/silicone serum
- separates serum from whole blood
What are the five reasons for a blood draw?
- Serum or plasma for chemical analysis
- serum or serology for analysis
- parasite analysis
What vacutainer does serum come form?
A red vacutainer being spun down
What is serum? "Pure portion"
Liquid portion of blood not containing WBC, RBC, or any clotting factors
What vacutainer does Plasma come from?
Plasma comes from lavender (purple) vacutainer spun down
What is plasma?
liquid portion of blood not containing RBC, WBC, does have clotting agents
Proper whole blood handling
- correct container
- gently invert 10-12 times and role in fingers
- never shakeif making slide for hand count make immediately
What should you label on your vacutainer?
- Iinitials (yours)
- Animals name (first and last)
- Location of blood draw
How long should you allow serum and plasma to clot?
How long and rpms should you centrifuge serum and plasma?
10 minutes at 800-1000 rpms