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Eukaryotic Virus Vectors
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The virion consists of:
a nucleic acid genome
a protective protein coat (capsid)
some can have a lipid envelope
What are viruses?
obligate intracellular parasites that can only replicate within a living cell
What do viruses lack?
many essential functions:
- synthesis of basic biological building blocks
- generation of ATP
- protein synthesis
What is the viral replication cycle?
1) binding to cell receptor
2) entry and uncoating
3) early gene expression
4) replication of viral genome
5) late gene expression
6) assembly of virions
7) exit
Many viruses can be what?
engineered to deliver and express specific genes
- Viral vectors are genetically modified to deliver and express their own or foreign genes
What are virus vectors used for?
production of specified proteins in cultured cells
vaccination of animals or humans against disease
gene therapy in animals or humans
anticancer agents that specifically kill tumor cells
How do you construct vectors?
Typical virus vectors are unable to replicate due to deletion of essential viral genes
1) Delete some or all virus genes
2) Insert transgenes
3) Transfect packaging cell line
4) Harvest recombinant virus
Author
DesLee26
ID
309904
Card Set
Eukaryotic Virus Vectors
Description
Test Two
Updated
10/25/2015, 4:25:02 PM
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