Eukaryotic Virus Vectors

Card Set Information

Author:
DesLee26
ID:
309904
Filename:
Eukaryotic Virus Vectors
Updated:
2015-10-25 12:25:02
Tags:
Genetics
Folders:
Zuzga
Description:
Test Two
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  1. The virion consists of:
    • a nucleic acid genome
    • a protective protein coat (capsid)
    • some can have a lipid envelope
  2. What are viruses?
    obligate intracellular parasites that can only replicate within a living cell
  3. What do viruses lack?
    • many essential functions: 
    • - synthesis of basic biological building blocks
    • - generation of ATP
    • - protein synthesis
  4. What is the viral replication cycle?
    • 1) binding to cell receptor
    • 2) entry and uncoating
    • 3) early gene expression
    • 4) replication of viral genome
    • 5) late gene expression
    • 6) assembly of virions
    • 7) exit
  5. Many viruses can be what?
    • engineered to deliver and express specific genes
    • - Viral vectors are genetically modified to deliver and express their own or foreign genes
  6. What are virus vectors used for?
    • production of specified proteins in cultured cells
    • vaccination of animals or humans against disease
    • gene therapy in animals or humans
    • anticancer agents that specifically kill tumor cells
  7. How do you construct vectors?
    Typical virus vectors are unable to replicate due to deletion of essential viral genes

    • 1) Delete some or all virus genes
    • 2) Insert transgenes
    • 3) Transfect packaging cell line
    • 4) Harvest recombinant virus

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