Eukaryotic Virus Vectors
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The virion consists of:
- a nucleic acid genome
- a protective protein coat (capsid)
- some can have a lipid envelope
What are viruses?
obligate intracellular parasites that can only replicate within a living cell
What do viruses lack?
- many essential functions:
- - synthesis of basic biological building blocks
- - generation of ATP
- - protein synthesis
What is the viral replication cycle?
- 1) binding to cell receptor
- 2) entry and uncoating
- 3) early gene expression
- 4) replication of viral genome
- 5) late gene expression
- 6) assembly of virions
- 7) exit
Many viruses can be what?
- engineered to deliver and express specific genes
- - Viral vectors are genetically modified to deliver and express their own or foreign genes
What are virus vectors used for?
- production of specified proteins in cultured cells
- vaccination of animals or humans against disease
- gene therapy in animals or humans
- anticancer agents that specifically kill tumor cells
How do you construct vectors?
Typical virus vectors are unable to replicate due to deletion of essential viral genes
- 1) Delete some or all virus genes
- 2) Insert transgenes
- 3) Transfect packaging cell line
- 4) Harvest recombinant virus
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