Lecture 14- techniques

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Author:
miingargiola
ID:
31140
Filename:
Lecture 14- techniques
Updated:
2010-08-24 20:10:05
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Lecture techniques
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Lecture 14- techniques
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  1. Southern Blotting
    • Cleave the DNA
    • with restriction enzymesSeparate
    • fragments on agarose gelTransfer
    • fragments to support, like nitrocellulose, in same positionHybridize with a
    • specific probe to detect certain DNA sequences/genes of interest
  2. Northern Blotting
  3. Similar to Southern Blotting, but bind and analyze RNA
  4. Western Blotting
    Separate the proteins by size (electrophoresis)

    Bind antibodies to find proteins of interest
  5. All techniques

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