Stage 2 of Glycolysis ( Glycolytic pathway)
Now we are going to break the Fructose 1,6 biphosphate that we made in stage one and break it up into two parts.Take this 6 carbon molecule and break it into two 3 carbon molecules each of which whill be carrying a phosphate. This is called a reverse aldol condensation. The enzyme that carries out this reaction is called Aldolase. This reaction is very reversible. but the products of this reaction is immediately going to be shuttled through the rest of this pathway, so because of the quickness of the shuttling it is unlikely that these products will reform F-1,6-BP. One of the products of this reaction- glyceraldehyde 3 phosphate(GAP) is the next substrate in the pathway. GAP goes on to be phosphorylated and generate energy in the form of ATP. dihydroxyacetone phosphate ( DHAP) is not the next substrate in this pathways thus it must be converted into glyceraldehyde 3 phosphate in order to move on in the pathway. We convert DHAP to GAP in a simple step celled triose phosphate isomerase. The next result of the aldorase reaction, the next result is the generation of two glyceeraldehy 3 phosphates.