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166 bp of DNA wrapped around the histone core and held in poace by H1 (a linker histone)
What is in situ hybridization?
- situ hybridization, also referred to a hybridization histochemistry, was introduced in
- 1969 (Buongiorno-Nardelli and Amaldi, 1969; John et al., 1969). The basic technique
- utilizes the fact that DNA and RNA will undergo hydrogen bonding to complimentary
- sequences of DNA or RNA. By labeling sequences of DNA or RNA of sufficient length
- (approximately 50-300 base pairs), selective probes can be made to detect particular
- sequences of DNA or RNA. The application of these probes to tissue sections allows DNA or
- RNA to be localized within tissue regions and cell types. In situ hybridization is
- a powerful technique and unique in the way that it allows one to study the macroscopic
- distribution and cellular localization of DNA and RNA sequences in a heterogeneous cell