Card Set Information
histones nucleosomes chromatin fibers DNA chromosomes
Structure of chromatin review
Function of histones
166 bp of DNA wrapped around the histone core and held in poace by H1 (a linker histone)
What is in situ hybridization?
hybridization, also referred to a hybridization histochemistry, was introduced in
1969 (Buongiorno-Nardelli and Amaldi, 1969; John et al., 1969). The basic technique
utilizes the fact that DNA and RNA will undergo hydrogen bonding to complimentary
sequences of DNA or RNA. By labeling sequences of DNA or RNA of sufficient length
(approximately 50-300 base pairs), selective probes can be made to detect particular
sequences of DNA or RNA. The application of these probes to tissue sections allows DNA or
RNA to be localized within tissue regions and cell types.
a powerful technique and unique in the way that it allows one to study the macroscopic
distribution and cellular localization of DNA and RNA sequences in a heterogeneous cell