Chromatin structure

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Chromatin structure
2010-08-25 23:00:44
histones nucleosomes chromatin fibers DNA chromosomes

Structure of chromatin review
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  1. Function of histones
  2. Chromatosome
    166 bp of DNA wrapped around the histone core and held in poace by H1 (a linker histone)
  3. What is in situ hybridization?
    • In
    • situ hybridization, also referred to a hybridization histochemistry, was introduced in
    • 1969 (Buongiorno-Nardelli and Amaldi, 1969; John et al., 1969). The basic technique
    • utilizes the fact that DNA and RNA will undergo hydrogen bonding to complimentary
    • sequences of DNA or RNA. By labeling sequences of DNA or RNA of sufficient length
    • (approximately 50-300 base pairs), selective probes can be made to detect particular
    • sequences of DNA or RNA. The application of these probes to tissue sections allows DNA or
    • RNA to be localized within tissue regions and cell types. In situ hybridization is
    • a powerful technique and unique in the way that it allows one to study the macroscopic
    • distribution and cellular localization of DNA and RNA sequences in a heterogeneous cell
    • population.