Biochem 3300 - Glycolysis I PII

The flashcards below were created by user Ant on FreezingBlue Flashcards.

  1. Why is phosphofructokinase-1 so essential to glycolysis?
    It calalyzes one of the rate determining steps
  2. Substrates and products of reaction 3
    • Substrate - Fructose-6-phosphate
    • Product - Fructose-1,6-bisphosphate
  3. What macronutrient is required for reaction 3?
    Mg2+
  4. ΔG'° of reaction 3
    -14.2 KJ mol-1
  5. What kind of reaction is reaction four?
    • Chemistry - Retro Aldol
    • Biochemistry - Class I Aldolase
  6. Substrates and products of reaction four?
    • Substrate - Fructose-1,6-bisphosphate
    • Product - Glyceraldehyde-3-phosphate and dihydroxyacetone phosphate
  7. ΔG'° of reaction 4
    -23.8 KJ mol-1
  8. Two key residues in reaction 4
    • Lysine (schiff base)
    • Aspartate
  9. Reaction 4's mechanism
    • 1. Lysine forms a schiff base with C2, C2's oxygen leaves as water
    • 2a. Withdrawal of electrons to NH+ causes bond C3-C4 to donate electrons to C2-C3 eliminating G3P
    • 2b. Aspartate abstracts a proton from C4-O-H forming C4=O
    • 3. HN:-C2=C3 tautomerize, aspartate donates its proton to C3
    • 4. The C2=NH+ schiff base is hydrolyzed completely forming dihydroxyacetone phosphate
  10. Reaction five substrate and products
    • Substrate: Dihydroxyacetonephosphate
    • Product: Glyceraldehyde-3-phosphate
  11. ΔG°' of reaction five
    7.5 KJ mol-1 (reversible)
  12. Intermediate of reaction five
    • Enediol
    • CH(OH)C(OH)CH2OPO32-
  13. What does the loop in triose phosphate isomerase do?
    • Hydrogen bonds with substrate phosphate group
    • Closes the pocket
    • Prevents side reactions
  14. What happens if TIM's loop is removed or damaged?
    Catalytic activity is significantly hindered
  15. What residues form TIM's loop?
    168-177
Author:
Ant
ID:
314999
Card Set:
Biochem 3300 - Glycolysis I PII
Updated:
2016-01-31 05:28:51
Tags:
Biochem 3300
Folders:

Description:
Biochem 3300
Show Answers: