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Why is phosphofructokinase-1 so essential to glycolysis?
It calalyzes one of the rate determining steps
Substrates and products of reaction 3
- Substrate - Fructose-6-phosphate
- Product - Fructose-1,6-bisphosphate
What macronutrient is required for reaction 3?
ΔG'° of reaction 3
-14.2 KJ mol-1
What kind of reaction is reaction four?
- Chemistry - Retro Aldol
- Biochemistry - Class I Aldolase
Substrates and products of reaction four?
- Substrate - Fructose-1,6-bisphosphate
- Product - Glyceraldehyde-3-phosphate and dihydroxyacetone phosphate
ΔG'° of reaction 4
-23.8 KJ mol-1
Two key residues in reaction 4
- Lysine (schiff base)
Reaction 4's mechanism
- 1. Lysine forms a schiff base with C2, C2's oxygen leaves as water
- 2a. Withdrawal of electrons to NH+ causes bond C3-C4 to donate electrons to C2-C3 eliminating G3P
- 2b. Aspartate abstracts a proton from C4-O-H forming C4=O3. HN:-C2=C3 tautomerize, aspartate donates its proton to C34. The C2=NH+ schiff base is hydrolyzed completely forming dihydroxyacetone phosphate
Reaction five substrate and products
- Substrate: Dihydroxyacetonephosphate
- Product: Glyceraldehyde-3-phosphate
ΔG°' of reaction five
7.5 KJ mol-1 (reversible)
Intermediate of reaction five
What does the loop in triose phosphate isomerase do?
- Hydrogen bonds with substrate phosphate group
- Closes the pocket
- Prevents side reactions
What happens if TIM's loop is removed or damaged?
Catalytic activity is significantly hindered
What residues form TIM's loop?