Bio 3400 - Lecture 2 PI

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  1. Types of compound light microscope
    • Bright-field
    • Dark-field
    • Phase contrast - Differences in density; live cells
    • Fluorescence
  2. Magnification formula
    Total magnification = object magnification x ocular magnification
  3. Regular light microscope lenses
    • Ocular
    • Objective
    • (specimen)
    • Condenser
  4. Magnification and resolution
    • Magnification - Make objects appear larger
    • Resolution - Distinguish adjacent points as separate objects
  5. Lenses in phase contrast microscope
    • Ocular
    • Diffraction plate
    • Objective lense
    • (specimen)
    • Condenser
    • Annular diaphragm
  6. Lenses in fluorescence microscope
    • Mercury arc lamp
    • Excitation filter (selects wavelength)
    • Dichroic mirror
    • Emission filter
  7. Differential interference microscopy
    • Polarizer created two distinct beams of polarized light
    • Gives structures 3D appearance
  8. Confocal scanning laser microscopy
    • Used lasers (ooh...)
    • Scan different layers, like MRI (Z-stacks)
    • 0.1um resolution
  9. Prokaryote size range
    • 0.2um to 700um
    • ¬†- Rods - 0.5um - 0.7um wide, 15um long
  10. Benefits of small cells
    • Greater S/V - Nutrient exchange
    • Faster growth
  11. Lower limit of cell size
    0.15um
  12. Phospholipid bilayer
    • 8-10nm wide
    • Hydrogen bond stabilization
    • Magnesium and Calcium ions stabillize membrane
  13. SusG
    Starch transport; moves freely - binds when starch is present
  14. Archeal membranes
    • Phospholipid ether linkages (ester in Bac and Euk)
    • Isoprenes (not fatty acids)
    • Tetraethers and glycerol
    • Monolayers
  15. Isoprene
    H2C=C(CH3)-CH=CH2
  16. Lipid monolayer
    single layer with hydrophillic heads attached to one hydrophobic chain
  17. Transport types
    • Simple - Driven by proton motive force
    • Group translocation - Chemical modification, PEP powered¬†
    • ABC - Periplasmic proteins, ATP powered
  18. ABC transport
    • ATP driven
    • Substrate specific
    • Uses periplasmic binding proteins
Author:
Ant
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Bio 3400 - Lecture 2 PI
Updated:
2016-02-03 22:22:02
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Bio 3400
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