Molec Cell Midterm 1
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Which 7 amino acids are hydrophobic?
What are amphipathic detergents used for?
to solubilize membrane proteins
Describe structure of a phospholipid
2 fatty acid chains attached to 2 carbons in a glycerol molecule, 3rd carbon attaches to a phosphate group which is attached to the polar head
Difference between micelles and liposomes
- micelles - one layer, hydrophobic tails pointing in
- liposomes - bilayered, there is a core inside
Method for analyzing membrane fluidity?
- Fluorescence Recovery After Photobleaching (FRAP)
- fluorescent proteins are spread out evenly throughout the membrane
- shine laser on membrane to bleach it so it doesn't fluoresce anymore
- recover fluorescence by moving around proteins = fluidity
Factors affecting fluidity?
- temperature - more heat = more fluid
- cholesterol - more cholesterol = less fluid
7 Universal properties of biological membranes
- sheet-like structures (lipid leaflets)
- contain mostly lipids & proteins
- both proteins & lipids mediate function
- non-covalent assemblies
- closed boundaries
- asymmetric (cytosolic face and exosplasmic face)
composition of phosphoglycerides (4 things)
- fatty acids
- phosphate group
- alcohol head group
Saturated vs unsaturated fatty acids - which is more stable / fluid?
- saturated - straight
- unsaturated - bent
- saturated fatty acids have higher stability but less fluidity
What does cholesterol do?
- stiffens the membrane
- extremely hydrophobic
3 types of membrane proteins
- lipid-anchor protein
- peripheral membrane protein
- integral membrane protein
What do GPI anchors do?
tether proteins to the exoplasmic face
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