Molec Cell Midterm 1

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  1. Which 7 amino acids are hydrophobic?
    • alanine
    • valine
    • isoleucine
    • leucine
    • methionine
    • phenylalanine
    • tyrosine
    • tytophan
  2. What are amphipathic detergents used for?
    to solubilize membrane proteins
  3. Describe structure of a phospholipid
    2 fatty acid chains attached to 2 carbons in a glycerol molecule, 3rd carbon attaches to a phosphate group which is attached to the polar head
  4. Difference between micelles and liposomes
    • micelles - one layer, hydrophobic tails pointing in
    • liposomes - bilayered, there is a core inside
  5. Method for analyzing membrane fluidity?
    • Fluorescence Recovery After Photobleaching (FRAP)
    • fluorescent proteins are spread out evenly throughout the membrane
    • shine laser on membrane to bleach it so it doesn't fluoresce anymore
    • recover fluorescence by moving around proteins = fluidity
  6. Factors affecting fluidity?
    • temperature - more heat = more fluid
    • cholesterol - more cholesterol = less fluid
  7. 7 Universal properties of biological membranes
    • sheet-like structures (lipid leaflets)
    • contain mostly lipids & proteins
    • both proteins & lipids mediate function
    • non-covalent assemblies
    • fluid
    • closed boundaries
    • asymmetric (cytosolic face and exosplasmic face)
  8. composition of phosphoglycerides (4 things)
    • glycerol
    • fatty acids
    • phosphate group
    • alcohol head group
  9. Saturated vs unsaturated fatty acids - which is more stable / fluid?
    • saturated - straight 
    • unsaturated - bent
    • saturated fatty acids have higher stability but less fluidity
  10. What does cholesterol do?
    • stiffens the membrane
    • extremely hydrophobic
  11. 3 types of membrane proteins
    • lipid-anchor protein
    • peripheral membrane protein
    • integral membrane protein
  12. What do GPI anchors do?
    tether proteins to the exoplasmic face

Card Set Information

Author:
st2478
ID:
317040
Filename:
Molec Cell Midterm 1
Updated:
2016-03-09 06:30:34
Tags:
bio midterm
Folders:
Molec Cell Bio
Description:
Lectures 1-11
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