Biology 3400 - Lecture 10

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  1. Selectable mutation
    • Provide growth advantage under certain conditions
    • Nonselectable (no advantage or disadvantage)
  2. Mutation types
    • Missense (change residue)
    • Nonsense (premature stop)
    • Silent (different codon, same residue)
  3. Revertant
    • Strain that has had its original phenotype reverted (restored)
    • Same site
    • Second site
  4. Nucleotide error rates
    Genomic DNA (x10^-~6) <<< Viral DNA <<< Genomic RNA
  5. Nonionizing mutagenesis
    Pyrimidine dimer (from UV)
  6. DNA repair
    • Direct reversal - recognizable mutation is quickly and easily fixed
    • Repair of ssDNA damage - Damaged DNA ia removed and replaced using opposite strand
    • Repair of dsDNA damage - SOS repair; translesion (no template); lex system
  7. Homologous recombination
    Genetic exchange of homologous DNA from two sources
  8. Transduction
    • DNA transfer by bacteriophage
    • Generalized - Random genomic DNA is packaged into virion
    • Specialized
  9. Phage conversion
    Alteration of the phenotype of a host cell by a lysogenization
  10. plasmid
    • Found in conjugating donar cells (sex pilus)
    • ~100kb
    • DNA replication genes
    • transposable elements for genomic integration (episome)
    • tra genes encode transfer functionality
  11. Hfr cells
    • High frequency recombination
    • Integrated F plasmid
    • F+ is nonintegrated
  12. F plasmid abilities
    • Synthesize F (sex) pilus
    • Horizontal gene transfer
    • Surface receptors block further reception
  13. F-plasmid insertsion sequences
    IS3 mobile elements
  14. Archael genome
    Single circular
  15. Transposon methods
    • Cut and paste
    • Copy and paste
  16. Insertion sequences
    • ~1000bp
    • 10-50bp inverted repeats
    • Found in bacterial and arachael plasmids

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Author:
Ant
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317130
Filename:
Biology 3400 - Lecture 10
Updated:
2016-03-10 05:00:33
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Biology
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Biology
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