Biochem 3300 - TCA Cycle II

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  1. Overall reaction of the TCA cycle
    • Reactants: 3NAD+ + FAD + GTP + Pi + Acetyl-CoA
    • Products: 3NADH + FADH + GTP + CoA +2CO2
  2. Replenishing reactions (word)
    Anaplerotic
  3. First three steps of TCA cycle
    • 1. Acetyl-Coa (+ Oxoalotacetate) -> Citrate
    • 2. Citrate -> Isocitrate
    • 3. Isocitrate -> α-ketoglutyrate
  4. Second three steps of the TCA cycle
    • 4. -ketoglutyrate -> Succinyl-Coa
    • 5. Succinyl-CoA -> Succinate
    • 6. Succinate -> Fumarate
  5. Third set of steps of TCA cycle
    • 7. Fumarate -> Malate
    • 8. Malate -> Oxaloacetate
  6. Citrate synthase mechanism
    • Make an enol intermediate
    • Join two compounds
    • Hydrolyse CoA-SH
  7. Isocitrate dehydrogenase
    • 1. Oxidize to ketone [NAD(P+)]
    • 2. CO2 leaves
    • 3. Add H+
  8. Nucleoside diphosphate kinase
    Converts GTP to ATP
  9. Key steps of succinyl-CoA synthetase
    • 1. Replace S-CoA with phosphate
    • 2. Phosphorylate enzyme-his
  10. Succinyl CoA synthetase structure
    • Two subunits
    • 1. α: His
    • 2. β: Confers triphosphate specificity
  11. Malonate
    Succinate analog inhibits succinate dehydrogenase
  12. Succinate dehydrogenase mechanism
    Use ion sulfur centres and FAD to form an alkene
  13. Succinate dehydrogenase location
    • Eukaryotes: Inner mitochondrial membrane
    • Prokaryotes: Plasma membrane
  14. Fumarase specificity
    Only trans bonds
  15. Mechanism of L-Malate dehydrogenase
    Oxidize with NAD to form ketone

Card Set Information

Author:
Ant
ID:
317329
Filename:
Biochem 3300 - TCA Cycle II
Updated:
2016-03-13 23:47:47
Tags:
Biochemistry
Folders:
biochemistry
Description:
Biochemistry
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