Biochem 3300 - OxPhos

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  1. Oxidation of NADH
    • NADH +(1/2)O2 -> NAD+ + H2O
    • E = 1.14V
  2. Free energy change of redox reactions
    ΔG = -nFΔE
  3. How many protons cross the membrane per electron pair?
    10
  4. Potential energies of the PMF
    • Chemical
    • Electrical
  5. Alternative methods of electron transport
    • Electrons transferred to O2
    • No proton translocation
    • Energy released as heat
  6. ATP Machine
    F1F0 ATP Synthase
  7. SMP
    • Submitochondrial particles
    • Created from mitochindrial inner membrane by ultrasound
    • Inside-out (F1 outside)
  8. Inhibitors of F1Fo
    • Oligomycin - Antibiotic
    • DCCD - Covalently links hydrophobic carboxyls
  9. F1 Subunits
    • 3α, 3β, γ (bent helical shaft), δ, ε
    • Catalysis at αβ interface (mostly β)
  10. F0 subunits
    • a, 2b, 10c
    • c is proton turbine
  11. Binding change mechanism states
    • Loose (Bind)
    • Tight (Catalyze)
    • Open (Release)
  12. J.E Walker
    Solved crystal structure of F1

Card Set Information

Author:
Ant
ID:
317455
Filename:
Biochem 3300 - OxPhos
Updated:
2016-03-16 21:01:35
Tags:
Biochem 3300
Folders:
Biochem
Description:
Biochem
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