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Background of Marburg Virus
- In 1967, 3 lab workers became ill after processing monkey organs in Marburg, Germany.
- 3 got a severe fever--> led to a small localized outbreak, 32 people caught it, and 7 died
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Relation of ebola and marburg
in the same family
ebola is also pretty closely related to rabies, measles, and mumps, as well as a distantly related flu virus
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Capsid of filviruses
Diameter determined by nucleocapsid and structure
filamentous envelope particles and a helical nucleocapsid
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Genome of filovirus
linear, ssRNA, negative sense, one segment
seven genes transcribed by viral RNA pol
One gene makes one RNA, which makes one single protein
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Genes and protins
Most proteins are packaged in the virion
- Nucleocapsid (NP)
- RNA polymerase cofactor (VP35)
- Matrix protein (VP 40)
- Envelope glycoproteins (GP, cleavd into GP1 and GP2)
- Minor nucleocapsid protein (VP30)
- Membrane protein (VP24)
- RNA polymerase (L)
Ebola makes additional secreted glycoproteins (sGP)
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Virion of the virus
the genome is not infectious by itself because it is negative sense
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Viruses and hosts?
These viruses are __. The outbreak pattern is probably from __ .
Two genera
Marburg has one species
Ebola has five species
zoonotic
fruit bats to primates
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Infections with __ and __ viruses cause __.
Major symptoms are __, __, __, __, and __.
Lethality can be __.
Vaccine?
Local outbreaks are often followed by __.
Marburg and Ebola viruses; severe hemorrhagic fever
fever, hemorrhages, liver dysfunction, intravascular coagulation, and shock
high (up to 90%)
no treatment or licensed vaccine
lengthy absence of disease
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Marburg and EBola viruses are __
sporadically emerging viruses that cause severe often fatal disease
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OUtbreak of Marburg?
first outbreak of Marburg virus was in Marburg and Germany in 1967
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Outbreak of Ebola
first outbreak in Zaire and Sudan in 1976
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Filoviruses are related to __ and __.
They cause __.
paramyxoviruses and rhabdoviruses
hemorrhagic fever
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__ is the only filovirus that has not been associated with human disease.
Reston ebolavirus
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Filovirus genomes contain __ in a __.
All genes are flanked by __ that signal __, __, and __.
seven genes
conserved order
- conserved sequences
- termination, polyadenylation, and reinitiation
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There are similarities to other mononegaviruses, such as the __.
the __ is at the very end.
There is a similar method of trascription, where __; and, each time, at the end of the gene, the polymerase __ so that you get more products of the proteins at the __ and
- NP protien
- polymerase
- single entry down at the 3'
- falls off
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