Microbiology Lab Exercises 6

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  1. a differential staining procedure is one wher more than one stain (and sometimes more than one type of stain) is used to differentiate bacteria into groups
    differential stain
  2. a staining technique for the preliminary identification of bacteria, in which a violet dye is applied, followed by a decolorizing agent and then a red dye.; differential staining
    gram stain
  3. stains bacterial cell
    Crystal Violet (primary stain)
  4. mordant, bind the stain
    Iodine
  5. differentiates bacteria by retaining or not crystal violet, within their cell wall
    Alcohol-acetone
  6. counter stain for some fastidious microorganisms. This will dye the colorless cells.
    Safranin
  7. diff between gram pos/neg bacteria
    gram positive cell walls will retain color, the gram neg walls can be dissolved by alcohol so they don't.
  8. what does the gram stain actually stain
    bacteria
  9. what is the most critical step in gram staining
    Decolorization. because it's easy to over decolorize or under decolorize the sample
  10. basic gram stain sequence
    crystal violet, iodine, ethenol, safranin.
  11. organisms used in this lab and its reaction
    e.coli- gram neg, pink; m. luteus-gram pos, purple

Card Set Information

Author:
hey_itsdarra
ID:
323709
Filename:
Microbiology Lab Exercises 6
Updated:
2016-09-27 03:05:31
Tags:
UofM microbiology lab
Folders:
UofM Microbiology Lab
Description:
exam 1
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