LAB 2 . IBHS

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What is genetic transformation?
- -a process that allows insertion of foreign DNA into cells.
- -literally, it means a change caused by genes. insertion of a gene to change organism's trait(s).
How do you genetically transform an organism?

ALL cells of that organism must be transformed (with the gene of interest)
Why use E. coli for genetic transformation in lab?
- 1. grow quickly (simple, only one cell)
- 2. easy to maintain (limited resources and space)
- 3. easy diets (simple broth (liquid) or agar (solid)).
What does Luria Bertani comprise of?
- AKA "LB" not to be confused with LC.
- 1. Amino acid
- 2. vitamins
- 3. minerals
- 4. NaCl
Plasmids

small circular pieces of DNA
T/F
plasmid DNA is useless in terms of benefiting bacterial survival.

false. it may be beneficial.
T/F
plasmids can be transferred for sharing.

TRUE :D yay i know you got it right.
gene

a piece of DNA that is the instructions for protein making.
Origin of Green Fluorescent Protein (GTP)

bioluminescent jellyfish - Aequorea victoria
Bacteria is green color under U.V.
transformed or not?

transformed
Where can the activity of the protein be controlled at?
- 1. transcriptional level (DNA to RNA)
- 2. translational level (RNA to protein)
- 3. post-translational (modifications)
T/F
gene expression (transcription and translation) doesn't require that much energy

FALSE. it requires significant energy input from a cell.
Why do we need to use inducible promotors?

to control gene regulation
5' UTR
- region that is non-coding (untranslated) upstream of start codon in DNA sequence.
- contains promotor

Author

VASUpharm14

32556

Card Set

LAB 2 . IBHS

Description

Lab 2: Recombinant DNA technology

Updated

2010-09-03T04:36:26Z

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