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VASUpharm14
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What is genetic transformation?
- -a process that allows insertion of foreign DNA into cells.
- -literally, it means a change caused by genes. insertion of a gene to change organism's trait(s).
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How do you genetically transform an organism?
ALL cells of that organism must be transformed (with the gene of interest)
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Why use E. coli for genetic transformation in lab?
- 1. grow quickly (simple, only one cell)
- 2. easy to maintain (limited resources and space)
- 3. easy diets (simple broth (liquid) or agar (solid)).
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What does Luria Bertani comprise of?
- AKA "LB" not to be confused with LC.
- 1. Amino acid
- 2. vitamins
- 3. minerals
- 4. NaCl
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Plasmids
small circular pieces of DNA
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T/F
plasmid DNA is useless in terms of benefiting bacterial survival.
false. it may be beneficial.
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T/F
plasmids can be transferred for sharing.
TRUE :D yay i know you got it right.
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gene
a piece of DNA that is the instructions for protein making.
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Origin of Green Fluorescent Protein (GTP)
bioluminescent jellyfish - Aequorea victoria
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Bacteria is green color under U.V.
transformed or not?
transformed
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Where can the activity of the protein be controlled at?
- 1. transcriptional level (DNA to RNA)
- 2. translational level (RNA to protein)
- 3. post-translational (modifications)
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T/F
gene expression (transcription and translation) doesn't require that much energy
FALSE. it requires significant energy input from a cell.
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Why do we need to use inducible promotors?
to control gene regulation
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5' UTR
- region that is non-coding (untranslated) upstream of start codon in DNA sequence.
- contains promotor
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