LAB 2 . IBHS
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What is genetic transformation?
-a process that allows insertion of foreign DNA into cells.
-literally, it means a change caused by genes. insertion of a gene to change organism's trait(s).
How do you genetically transform an
ALL cells of that organism must be transformed (with the gene of interest)
for genetic transformation in lab?
1. grow quickly (simple, only one cell)
2. easy to maintain (limited resources and space)
3. easy diets (simple broth (liquid) or agar (solid)).
What does Luria Bertani comprise of?
AKA "LB" not to be confused with LC.
1. Amino acid
small circular pieces of DNA
plasmid DNA is useless in terms of benefiting bacterial survival.
false. it may be beneficial.
plasmids can be transferred for sharing.
TRUE :D yay i know you got it right.
a piece of DNA that is the instructions for protein making.
Origin of Green Fluorescent Protein (GTP)
bioluminescent jellyfish -
Bacteria is green color under U.V.
transformed or not?
Where can the activity of the protein be controlled at?
1. transcriptional level (DNA to RNA)
2. translational level (RNA to protein)
3. post-translational (modifications)
gene expression (transcription and translation) doesn't require that much energy
FALSE. it requires
input from a cell.
Why do we need to use inducible promotors?
to control gene regulation
region that is non-coding (untranslated) upstream of start codon in DNA sequence.
LAB 2 . IBHS
Lab 2: Recombinant DNA technology