M05 - Enterobacteriacae and Biochemical Test Media

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  1. A positive DNase test would be seen with

    A) Serratia marcescens
    B) Klebsiella oxytoca
    C) Proteus mirabilis
    D) Escherichia coli
    A) Serratia marcescens
    (this multiple choice question has been scrambled)
  2. Which of the following growth characteristics is typical for Escherichia coli?

    A) Beta-hemolytic on sheep blood agar (BAP)
    B) Colorless colonies on MacConkey agar
    C) Colorless colonies on xylose-lysine-desoxycholate (XLD) agar
    D) Green colonies with black centers on Hektoen enteric (HE) agar
    A) Beta-hemolytic on sheep blood agar (BAP)
    (this multiple choice question has been scrambled)
  3. Which of the following organisms produce a positive phenylalanine deaminase reaction?

    A) Proteus mirabilis
    B) Morganella morganii
    C) All of the above
    D) Providencia stuartii
    C) All of the above
    (this multiple choice question has been scrambled)
  4. Identify the Gram-negative bacillus that can be described as:
    lactose: negative
    oxidase: negative
    indole: negative
    citrate: positive
    methyl red: positive
    urease: negative
    H S: positive
    clear colonies with black center on XLD

    A) Salmonella enteritidis.
    B) Klebsiella pneumoniae
    C) Escherichia coli.
    D) Shigella sonnei
    A) Salmonella enteritidis.
    (this multiple choice question has been scrambled)
  5. Which species of Shigella is most commonly associated with diarrheal disease in the USA?

    A) flexneri
    B) sonnei
    C) dysenteriae
    D) boydii
    B) sonnei
    (this multiple choice question has been scrambled)
  6. Shigella sp are genetically related to Escherichia coli but can easily be distinguished from each other using certain biochemical tests. List two biochemical tests you could perform to help you differentiate between these two organisms. Discuss the reactions you would expect to see for Shigella sp versus Escherichia coli for the biochemical tests you choose. *Note: ONE of your biochemical tests can be selective/differential plate media.
    Shigella can be differentiated from E.coli with HE agar plates. E. coli's ability to ferment lactose would produce acid and cause oranging of the agar while Shigella would not. Shigella from E. coli can also be differentiated using the TSI test as the shigella result would be K/A, alkaline over acid as it utilizes the peptones and E. coli would be A/A plus gas as it ferments the carb's producing acid and gas.
  7. A leg wound culture from a hospitalized 70-year-old diabetic man grew motile, lactose-negative colonies on MAC. Given the following biochemical reactions at 24 hours, what is the most probable organism?
    H S: negative
    Indole: negative
    MR/VP: negative/positive
    phenylalanine deaminase: negative
    DNase: positive
    citrate: positive
    urease: negative
    ornithine decarboxylase: positive
    lysine decarboxylase: positive
    arginine dehydrolase: negative
    Gelatin hydrolysis: negative

    A) Escherichia coli
    B) Enterobacter cloacae
    C) Serratia marcescens
    D) Klebsiella pneumoniae
    C) Serratia marcescens
    (this multiple choice question has been scrambled)
  8. What amino acid is the substrate in indole test broth?

    A) Arginine
    B) Tryptophan
    C) Ornithine
    D) Leucine
    B) Tryptophan
    (this multiple choice question has been scrambled)
  9. Which genera of Enterobacteriaceae are usually nonmotile at 35°C?

    A) Serratia, Morganella, and Shigella
    B) Shigella, Yersinia, and Salmonella
    C) Shigella, Klebsiella, and Yersinia
    D) Shigella, Edwardsiella, and Enterobacter
    C) Shigella, Klebsiella, and Yersinia
    (this multiple choice question has been scrambled)
  10. (T/F) The Moeller Decarboxylase Test requires an overlay of mineral oil to provide an anaerobic environment for decarboxylation, or hydrolysis of the amino acids to occur.
    True
  11. Three blood cultures taken from a 30-year-old cancer patient receiving chemotherapy and admitted with a UTI grew "swarming" colonies of lactose-negative, motile, Gram-negative rods prior to antibiotic therapy. Given the following biochemical reactions, which is the most likely organism?
    H S: positive
    indole: negative
    MR/VP: positive/negative
    phenylalanine deaminase: positive
    DNase: negative
    citrate: positive
    urease: positive (rapid)
    gelatin hydrolysis: positive
    ornithine decarboxylase: positive

    A) Proteus vulgaris
    B) Proteus mirabilis
    C) Providencia rettgeri
    D) Morganella morganii
    B) Proteus mirabilis
    (this multiple choice question has been scrambled)
  12. A number of vacationers who traveled outside the U.S. have had their vacations interrupted by a case of "traveler's diarrhea," which is commonly associated with which etiologic agent?

    A) Enterotoxigenic E. coli (ETEC)
    B) Proteus mirabilis
    C) Vibrio parahemolyticus
    D) Uropathogenic E. coli (UPEC)
    A) Enterotoxigenic E. coli (ETEC)
    (this multiple choice question has been scrambled)
  13. The medium used to determine whether an organism is oxidative or fermentative with respect to its metabolic activities is

    A) XLD medium
    B) CTA medium
    C) OF medium
    D) HE medium
    C) OF medium
    (this multiple choice question has been scrambled)
  14. Four blood cultures were taken over a 24-hour period from a 20-year-old woman with severe diarrhea. The cultures grew motile Gram negativerods. A urine specimen obtained by catheterization also showed Gram-negative rods, 100,000 col/mL. Given the following results, which is the most likely organism?
    TSI: A/A +gas
    Indole: positive
    MR/VP: positive/negative
    H S: negative
    citrate: negative
    urease: negative
    lysine decarboxylase: positive
    phenylalanine deaminase: negative

    A) Edwardsiella tarda
    B) Escherichia coli
    C) Serratia marscens
    D) Enterobacter aerogenes
    B) Escherichia coli
    (this multiple choice question has been scrambled)
  15. (T/F) The purpose of the inverted Durham tube placed inside Carbohydrate Fermentation test tubes is to identify which organisms can grow anaerobically inside the Durham tube.
    False
  16. Which test is used to presumptively identify verotoxin-producing Escherichia coli such as O157:H7.

    A) MUG
    B) Nitrate
    C) Oxidase
    D) ONPG
    A) MUG
    (this multiple choice question has been scrambled)
  17. The most appropriate biochemical test to differentiate between K. pneumoniae and K. oxytoca is

    A) Urea
    B) Voges-Proskauer
    C) Indole
    D) Motility
    C) Indole
    (this multiple choice question has been scrambled)
  18. Cefsulodin-irgasan-novobiocin (CIN) agar is recommended for the recovery of

    A) Salmonella
    B) Proteus mirabilis
    C) Enterobacter aerogenes
    D) Yersinia enterocolitica
    D) Yersinia enterocolitica
    (this multiple choice question has been scrambled)
  19. Which of the following organisms is considered an "anaerogenic biotype of E. coli?"

    A) EIEC & ETEC
    B) EHEC
    C) UPEC
    D) Shigella spp
    D) Shigella spp
    (this multiple choice question has been scrambled)
  20. Which pathogen can exhibit the stain characteristics shown in the photograph
    Image Upload



    A) Salmonella spp
    B) Diarrheagenic E. coli
    C) Yersinia spp
    D) Shigella spp
    C) Yersinia spp
    (this multiple choice question has been scrambled)
  21. A foot wound from a nursing home patient was cultured and grew on MAC as pink to dark pink, oxidase-negative mucoid colonies. Further results were:
    Indole: negative
    MR/VP: negative/postiive
    Citrate: positive
    Urease: positive
    Motility: negative
    H S: negative

    The most likely organisms is:

    A) Proteus vulgaris
    B) Enterobacter cloacae
    C) Serratia marcescens
    D) Klebsiella pneumoniae
    D) Klebsiella pneumoniae
    (this multiple choice question has been scrambled)
  22. Which of the following media and/or biochemical tests may be used to distinguish E.coli O157:H7 from commensal E.coli?

    A) HE and indole
    B) TSI and MAC
    C) MAC and SMAC
    D) SMAC and indole
    C) MAC and SMAC
    (this multiple choice question has been scrambled)
  23. Plesiomonas shigelloides is a relatively new member of the Enterobacteriaceae. What characteristic separates it from other members of the Enterobacteriaceae?

    A) it ferments lactose
    B) it is oxidase positive
    C) it requires 10% CO2 for growth
    D) it produces pyocyanin
    B) it is oxidase positive
    (this multiple choice question has been scrambled)
  24. A 64-year-old male with lymphoma has a positive blood culture at 18 hours incubation. The organism is a non-lactose fermenting Gram negative bacillus on MAC. Further testing gives the following reactions:
    oxidase=negative
    motility=positive
    indole=positive
    MR/VP=positive/negative
    citrate=positive
    urease=positive
    ornithine decarboxylase=negative
    phenylalanine deaminase=positive

    The genus is:

    A) Enterobacter
    B) Salmonella
    C) Edwardsiella
    D) Providencia
    D) Providencia
    (this multiple choice question has been scrambled)
  25. Infection with the bubonic form of plague disease occurs by which route?

    A) flea bite
    B) inhalation of contaminated airborne droplets
    C) ingestion of contaminated tissue
    D) fecal/oral
    A) flea bite
    (this multiple choice question has been scrambled)
  26. The Methyl Red test detects which end product of glucose fermentation?

    A) hydrogen sulfide
    B) acetoin
    C) mixed acids
    D) nitrite
    C) mixed acids
    (this multiple choice question has been scrambled)
  27. What substance is added to triple sugar iron (TSI) to detect the production of hydrogen sulfide gas?

    A) Copper sulfate
    B) Bromthymol blue
    C) Ferrous sulfate
    D) Peptones
    C) Ferrous sulfate
    (this multiple choice question has been scrambled)
  28. Match the statement from the left column with the best corresponding organism(s) in the right column.

    +Enterotoxigenic E. coli (ETEC)
    +Shigella infection
    +Salmonella enterica ss typhi
    +Enterohemorrhagic E. coli (EHEC)
    +Klebsiella spp.
    +Uropathogenic E. coli

    -Can lead to hemolytic uremic syndrome (HUS), produces Shiga toxin
    -Scant stool with blood, mucus and pus
    -Traveler's diarrhea
    -Major nosocomial pathogen; non-motile
    -Can lead to hemolytic uremic syndrome (HUS), produces Shiga toxin
    -Associated with eggs, dairy products, and cold-blooded animals
    -Iron chelation
    • Enterotoxigenic E. coli (ETEC): Traveler's diarrhea
    • Shigella infection: Scant stool with blood, mucus and pus
    • Salmonella enterica ss typhi: Associated with eggs, dairy products, and cold-blooded animals
    • Enterohemorrhagic E. coli (EHEC): Can lead to hemolytic uremic syndrome (HUS), produces Shiga toxin
    • Klebsiella spp.: Major nosocomial pathogen; non-motile
    • Uropathogenic E. coli: Iron chelation
  29. Which of the pathogenic E.coli is known for its stacked brick colony formation on the intestinal mucosa?

    A) EIEC
    B) ETEC
    C) EAEC
    D) EHEC
    E) DAEC
    F) EPEC
    C) EAEC
    (this multiple choice question has been scrambled)
  30. Yersinia pestis is characteristically

    A) Hydrogen sulfide positive
    B) Oxidase positive
    C) Urease negative
    D) Motile at 20-25 degrees C
    C) Urease negative
    (this multiple choice question has been scrambled)
  31. Which of the following organisms would produce a yellow slant and a yellow butt plus gas on TSI agar after incubating 18 hours?

    A) Salmonella
    B) Proteus mirabilis
    C) Serratia marcescens
    D) Escherichia coli
    D) Escherichia coli
    (this multiple choice question has been scrambled)
  32. The K antigen of the family Enterobacteriacea is

    A) The somatic antigen
    B) The antigen used to group Shigella
    C) Located on the flagellum
    D) Heat labile
    D) Heat labile
    (this multiple choice question has been scrambled)
  33. The majority of clinical isolates of Klebsiella are

    A) K. pneumoniae
    B) K. ozaenae
    C) K. oxytoca
    D) K. aerogenes
    A) K. pneumoniae
    (this multiple choice question has been scrambled)
  34. Which of the following is not true of Shigella?

    A) Severe symptoms include rectal prolapse and hemolytic uremic syndrome.
    B) The organism produces an inflammatory condition in the large intestine with bloody diarrhea.
    C) The organism grows as colorless colonies on MAC.
    D) Large numbers of organisms must be ingested to produce disease.
    D) Large numbers of organisms must be ingested to produce disease.
    (this multiple choice question has been scrambled)
  35. Hemolytic uremic syndrome is a complication after infection with

    A) Salmonella Typhi
    B) Vibrio cholerae O1
    C) E. coli O157:H7
    D) Yersinia enterocolitica
    C) E. coli O157:H7
    (this multiple choice question has been scrambled)
  36. Which member of the Enterobacteriaceae is a causative agent of caseous swellings called pseudotubercules?

    A) Yersinia pestis
    B) Yersinia pseudotuberculosis
    C) Proteus mirabilis
    D) Salmonella Typhi
    B) Yersinia pseudotuberculosis
    (this multiple choice question has been scrambled)
  37. One of the most common etiologic agents of community-acquired uncomplicated cases of cystitis is

    A) Enterobacter aerogenes
    B) Escherichia coli
    C) Klebsiella pneumoniae
    D) Proteus vulgaris
    B) Escherichia coli
    (this multiple choice question has been scrambled)
  38. Reptiles kept as pets are sometimes associated with the transmission of

    A) Campylobacter
    B) Salmonella
    C) Yersinia enterocolitica
    D) Serratia marcescens
    B) Salmonella
    (this multiple choice question has been scrambled)
  39. (T/F) These organisms are always considered pathogens, not commensal organisms, when they are isolated from patient specimens: Salmonella, Shigella, Yersinia.
    True
  40. Which test is performed on isolates of Klebsiella pneumoniae to identify if the isolate is a potential cause of pyogenic liver abscesses

    A) Indole test
    B) MUG test
    C) String test
    D) Oxidase test
    C) String test
    (this multiple choice question has been scrambled)

Card Set Information

Author:
victimsofadown
ID:
327394
Filename:
M05 - Enterobacteriacae and Biochemical Test Media
Updated:
2017-01-14 05:06:52
Tags:
M05 Enterobacteriacae Biochemical Test Media
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TESTS,M
Description:
M05 - Enterobacteriacae and Biochemical Test Media
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