MBio Metabolism I

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  1. What type of cell produces endospores
  2. Endospores are resistant to
    • Temperature
    • Dessication
    • UV
    • Chemicals
  3. What triggers endospore production
    Nutrient limitations
  4. Endospore layers
    • Core wall
    • Cortex
    • Spore coat
    • Exposporium
  5. Where is dipicolinic acid found, what is it for?
    • Spore coat
    • Binds water causing dehydration of the spore
  6. Stages of spore synthesis
    • A vegetative cell detects nutrient limitations
    • Asymmetric division commits cell to sporulation
    • A prespore is formed, that is separated by a septum
    • The prespore is engulfed
    • The cortex forms around the spore
    • The cell is lysed, releasing the spore
  7. Role of Acid soluble proteins in spores
    Protect from desiccation and UV radiation
  8. What is a spore's cortex composed of?
  9. C. diff
    Clostridium difficile
  10. Botulin bacteria
    Clostridium botulinum
  11. Anabolic
    Making things
  12. Catabolic
    Breaking things
  13. Reversible metabolic pathway
  14. Two main cellular molecules (by wet weight)
    • Water (70%)
    • Protein (20%)
  15. % Dry weight of cellular protein
  16. Starch homolog
  17. Parts of the bacteria cell containing protein
    • Ribosomes
    • Cytosol
    • Cell wall
    • Pili
  18. Parts of the bacteria cell containing RNA
    • Cytosol
    • Ribosomes
  19. Parts of the bacteria cell containing lipids
    • Cell membrane
    • Cell envelope
  20. Macronutrients
    C, N, P, S, Mg, Ca, Na, Fe, H, O
  21. Micronutrients
    Cr, Co, Cu, Mn, Mb, Ni, Se, Va, Zn, Fe
  22. Cellular uses of chelating agents
    Acquisition of trace minerals
  23. Culture medium, singular or plural?
    Singular. Plural is media
  24. Types of culture media
    • Defined - Exact composition known
    • Complex - Exact composition unknown, often extracts
  25. Benefit of liquid over solid (gel) media?
    Liquid allows for greater volume
  26. Media solidifying agent
  27. Advantage of solid media
    Allows observation of colony morphology and pigmentation
  28. Selective media
    Hostile for non-selected organisms
  29. Differential media
    Produce an observable change if specific conditions are met
  30. Selective components of MacConkey media
    Bial salts and crystal violet select against gram positive organisms
  31. Fastidious microorganisms
    • Organisms that have difficult growth requirements
    • Princess microbes
  32. How does MacConkey media act as a differential media
    • Lactose fermentation reduces the local pH
    • The reduced pH causes methyl red to change colour
  33. Mannitol salt agar
    • 7.5-10% NaCl
    • Phenol red indicator (yellow colouration)
  34. Carboxymethylcellulose stain
    Congo red
  35. Enrichment
    • Like selective media, but does not involve media composition
    • I.e temperature, nitrogen, etc
  36. Heliobacter pylori
    • Bacteria that like low pH
    • Found in the stomach
  37. Types of heterotrophic metabolism
    • Respiration
    • Fermentation
  38. Final electron acceptor in respiration
  39. Final electron acceptor in fermentation
  40. Electron acceptors in anaerobic respiration
    NO3-, Fe3+, SO42-, CO3-
  41. High energy compounds
    • PEP
    • ATP
    • Glucose 6 Phosphate
    • Acyl Phosphate
  42. Steps in respiration
    • Glycolysis
    • Kreb's cycle
    • ETP + OxPhos
  43. Alternative name for glycolysis
    Embden-Meyerhoff pathway
Card Set:
MBio Metabolism I
2017-02-18 19:20:02
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