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B cell surface immunoglobulins?
IgG, IgD
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Classical vs alternative complement pathway
- Classical: IgM/IgG activation
- In order except C1->C4->C2
- Alternative: polysaccharide activation
- begins with C3 (seen twice)
- BOTH PATHWAYS MEET AT C3 -->C5 --> C5b attack complex
- *C_a to plasma and C_b to cell except C2
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Describe inhibition testing
- (sera+Ab) -> +indicator Ag
- NO RESPONSE = positive (the Ab reacted with the sera)
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clinical sens. and spec.
- sens: TP/(TP+FN) x100
- seNsitivity looks for false Negatives
- spec: TN/(TN+FP) x100
- sPecificty looks for false Positives
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REMEMBER about these diseases - Rheumatoid arthritis, mono, celiac
- Rheu: anti IgG (RF = IgM anti-IgG, but can be IgG anti-IgG), anti CCP
- Mono: EBV, anti VCA=acute, anti EBNA=past inf
- EBNA = Epstein Barr Never Again
- celiac: anti-tTGA (tissue transglutaminase)
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ANA: pattern -> Ab -> disease
- Peripheral (rim) -> anti-dsDNA -> SLE
- Homogeneous (diffuse) -> anti-histone/DNA -> RA, SLE
- Speckled -> Anti- lots of stuff -> SLE, PM/DM, PSS
- Centromere -> anti-centromere -> CREST
- Nucleolar -> anti-nucleolar -> SLE & PSS
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HIV conf
- Wester blot
- + if 2/3 p24, gp41, gp120/160
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Ab to what virus DOES NOT prevent reinfection?
CMV
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Viral hepatitis Abs
- anti-HBs = immune via vaccine
- anti-HBs & anti-HBc = immune via vaccine
- think digging "Surface(Ag), Earth(Ag), Core(Ab), Earth(Ab), Surface(Ab)
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