GMan - Eukaryotic Replication

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  1. Approximate number of eukaryote origins of replication
    >25000
  2. Yeast DNA replication origins
    • Autonomously replication sequences (ARS)
    • ~160bp
  3. ARS Domains (sequences)
    • A   - Origin recognition
    • B1 - Origin recognition
    • B2 - Initiate replication
    • B3 - Initiate replication
  4. ORC
    • Origin Recognition complex
    • Composed of six proteins
    • Permanently bound (through cell cycle)
  5. Protein responsible for DNA melting in yeast
    • ABF1
    • (Binds B3)
  6. Mammalian helicase loading proteins
    • Cdc6
    • Cdt1
  7. Equivalent (functionally) prokaryote and eukaryote replication proteins
    • Prokaryote | Eukaryote
    • --------------|------------
    • SSB            | Replication Protein A
    • Beta-clamp | Proliferating cell nuclear Antigen
    • Υ-Complex  | Replication factor C
    • DNA gyrase | Topoisomerase II
    • Primase      | DNA Polymerase alpha
  8. Polymerases responsible for replicaiton of nuclear DNA
    • Polymerase alpha (primase)
    • Polymerase delta (lagging strand)
    • Polymerase epsilon (Leading strand)
  9. Polymerases responsible for genome maintenance
    • Polymerase beta
    • Polymerase gamma
  10. Polymerases with 3' to 5' exonuclease activity
    δ, ε, γ
  11. Mitochondrial polymerase
    γ
  12. Polymerase α, ε; number of subunits and role
    • 4
    • Primer synthesis (α)
    • Leading strand synthesis + excision repair (ε)
  13. Polymerase β; number of subunits and role
    • 1
    • Base excision repair
  14. polymerase γ, δ; number of subunits
    • 3
    • Sometimes 2 for δ


    • Mitochondrial DNA repair
    • Lagging strand synthesis + base excision
  15. What does MCM stand for?
    Mini chromosome maintenance proteins
  16. How many MCM subunit proteins are there?
    Six (numbered 2-7)
  17. MCM protein structure
    Closed - Ring of six subunits, biting eachother's tails
  18. When are MCM proteins loaded/activated
    • G1 Phase (Inactive)
    • Activated during S-phase
  19. Where do MCM proteins interact with the DNA
    At an origin, where they behave as one of the helicases
  20. Ubiquinylation proteins
    • CRL-Skp4 - (Cdt1)
    • CRL-Cdt2 - (ORC and Cdt1)
  21. Kinases
    • CcnA-Cdk(1 and 2)
    • DDK
    • Chk

Card Set Information

Author:
Ant
ID:
329530
Filename:
GMan - Eukaryotic Replication
Updated:
2017-03-15 02:58:42
Tags:
GMan
Folders:
GMan
Description:
GMan
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