PrevMed3- Food Safety Cases
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What are signs of shiga-toxin producing E coli? (5)
- diarrhea (bloody)
- severe stomach cramps
- +/- fever
- [in YOPI], hemolytic uremic syndrome
What is the incubation period of shiga-toxin producing E coli?
What is the major reservoir for shiga-producing E coli? What produce is commonly implicated?
- Cattle- reservoir
- Sprouts, leafy greens- produce
Shiga-toxin producing E coli is defined as ___________ by the USDA.
adulterant (zero tolerance policy)
What is the role of the local and state health departments in detecting outbreaks?
identify and investigate the majority of outbreaks
What is the role of the CDC in detecting outbreaks?
coordinated multi-staate outbreak investiagtions
What is pulsed field gel electrophoresis?
- whole genome fingerprint
- restriction enzyme cuts chromosome intro fragments, and separation of fragments using pulsed field results in the "fingerprint"
Detecting outbreaks is the result of...
isolation of a rare subtype at an unexpected frequency with pulsed field gel electrophoresis
What is the role of whole genome sequencing?
- rapidly overtaking other techniques for strain differentiation
- more precise identification of outbreak-associated strains
Why did it take so long to identify the chipotle E coli outbreak?
Patient must seek medical attention--> doctor must make correct diagnosis and submit a sample--> clinical lab must recover the pathogen and send it to the public health lab--> the public health lab must subtype and isolate then report the results--> the health dept must investigate the outbreak
What is the role of the FDA in investigating an outbreak?
ingredient trace-back investigation for FDA regulated products
What is the role of the USDA in the investigation of a food-borne illness outbreak?
regulations classify E coli as an adulterant
What are the most common symptoms of Salmonellosis in humans? (3)
- Diarrhea- bloody, mucousy
- [usually self-limiting]
Who has regulatory authority over poultry products?
USDA FSIS (Salmonella is not considered an adulterant)
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