Micro LAB

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  1. What is the function of the base?
    Arm of the microscope?
    • Serves as support, holds the illuminator 
    • Provides support between the tube and the base; used also for support when carried.
  2. What is the function of the stage?
    Stage adjustment knobs of the microscopejQuery1101005363825844015535_1507005532112
    • A platform for holding slides; has a center hole to permit light from entering from below into the lenses; mechanical stage in vertical and horizontal directions 
    • Used to move the slide on the mechanical stage in a vertical and horizontal directions
  3. What is the illuminator ?
    Condenser of the microscope?
    • Light source; at the base 
    • Collects and concentrats light upward through the object on stage
  4. What is the iris diaphragm?
    What is the Rheostat control knob?
    • Adjusts the amount of light emitting by the bulb
    • Controls the amount of light emitted by he bulb
  5. Function of the tube 
    Rotating nosepiece functions?
    • Holds the ocular lenses at the top end and the rotating nosepeice with objective lenses at the lower end 
    • Hold the objective lenses, which are rotated by means of the knurled ring
  6. Which magnification in lab is used to provide the greatest detail?
    Oil immersion
  7. In an event of an accidental spill or accident in the lab, what would be the appropriate action to take?
    Report any minor or major accident/spill to your instructor immediately
  8. What is the correct sequence for the Gram stain procedure?
    Crystal Violet, Iodine, Decoolorizer (Alcohol), Safranin
  9. What is the function of the iodine in the Gram stain procedure?
    Iodine serves as a mordant by binding to the crystal violet. An insoluble iodine-crystal violet complex is formed that allows gram positive bacteria to remain purple after this step in the gram staining procedure.
  10. What is the advantage of a wet prep?
    Advantage is being able to view live organisms along with motility, undistorted cell grouping, and of individual cell size and shape.
  11. 11. Gram positive cocci in clusters are usually associated with which organisms?
    Staphylococcal infections (Staphylococci
  12. 12. Gram negative organisms high lipid content in their cell wall does what when alcohol is used in the Gram stain procedure?
    The lipids dissolve in the decolorizer (alcohol) washed away along with dye.
  13. 13. What two conditions may cause gram variable staining (both positive and negative reactions)?
    • Bacterial cultures age and the wall begins to disintegrate, gram-positive cells may not fully retain the crystal violet-iodine complex and will appear falsely gram-negative or as a mixture of gram-positive and gram-negative. (Gram variable)
    • -1) cultures that have been incubated longer than 24 or 48 hours or patients specimens that are not examined within a few hours of collection.
    • 2) Smears that have been decolorized for too long
    • 3) Patients on antibiotics will stain wrong
  14. What type of stain is methylene blue and what is it used for?
    Acid Fast Stains, it is a secondary stain
  15. The basic cell grouping of cocci bacteria are?
    Bacteria have three basic shapes: Cocci - spherical (round), Bacillus- rod, Spirillum- spiraled.
  16. The basic shapes of microorganisms are?
    Bacteria have three basic shapes: Cocci - spherical (round), Bacillus- rod, Spirillum- spiraled.
  17. 18. What is pleomorphism?
    Bacteria that characteristically vary in their shape (size&Length) are pleomorphic ( pleo meaning " many" and morphic referring to shape.
  18. What are palisade shapes?
    line up in bundles of parallel bacilli, others form V,X, orY figures divide and split.
  19. 20. In the MTS Lab Training Program- urinary tract infections are usually cause by which organism?
  20. 21. In the MTS-bacterial meningitis in adults is usually cause by which organism?
    In children?
    In infant?
    • Streptococcus pneumonia
    • Neisseria meningitidis
    • Haemophilus influenza
  21. In the MTS- hospital acquired infections is usually cause by which organism?
    Staphylococus aureus
  22. Which organisms usually cause viral meningitis ?
    • Non-enveloped RNA viruses- members of the enterovirus subgroup of picornavirus- are responsible for at least half of the cases of viral meningitis.
    • Of these the most common offenders are coxasckie viruses, which can cause throat and chest pain, and echoviruses, which can cause rash

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Micro LAB
2017-10-03 05:04:13

Lab test 1
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