Enzyme Kinetics
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steady-state kinetics
[ES] constant rate of formation=rate breakdown of [ES] complex
what does Km=
(k
_{2 }
+ K
_{-1}
)/ k
_{1}
Michaelis Menton Equation
V
_{o }
= V
_{max }
[S]/(K
_{m}
+ [S])
K
_{cat}
=
V
_{max}
/[E
_{t}
]
BiBi rxns
ordered bibi
random bibi
ping-pong mech
Types of Enzyme Inhibition
Reversible
-Competitive
-Uncompetitive
-Mixed
Irreversible
-Noncovalent
-Covalent
Competitive inhibitor
Inhibitor binds to the enzyme active site and prevents binding of S and vice versa
what is α?
1 + [I]/K
_{I}
Michaelis-Menton Equation Competitive inhibition
Vo = Vmax [S]/(Kmα + [S])
A competitive inhibitor...
affects K
_{m}
not V
_{max}
Uncompetitive inhibition
I binds only to ES complex
An uncompetitive inhibitor ...
lowers V
_{max }
and K
_{m}
Michaelis-Menton Equation uncompetitive competition
Vo = Vmax [S]/(Km + [S]α')
Mixed Inhibition
Inhibitor binds to both the E and ES at a site distinct from where S binds
Michaelis-Menton Equation Mixed Inhibition
Vo = Vmax [S]/(Kmα + [S]α')
A mixed inihibitor
lowers Vmax
Apparent Vmax Competitive Inhibitor
V max
Apparent Km Competitive Inhibitor
αKm
Apparent Vmax Uncompetitive Inhibitor
Vmax/α'
Apparent Km Uncompetitive Inhibitor
Km/α'
Apparent Vmax Mixed Inhibitor
Vmax/α'
Apparent Km for Mixed Inhibition
αKm/α'
Irreversible Inhibition
Affects Km and Vmax
destroys enzyme
Noncovalent (transition state analogs)
Covalent modification (special classes)
Suicide Inactivators
Covalent irreversible inhibitor
Simplifying assumptions of MM treatment
K-2 insignificant
[P] insiginificant
V=k2[ES] but hard to measure k2 or [ES]
steady state assumption haldane
Pre-Steady State Kinetics
Single turnover
Rapid Mixing/sampling
typical enzyme in MM treatment
Rxn vs[S]
Saturation Kinetics
can be explained by ES
how to find Vmax and Km from reciprocal plot
slope=Km/Vmax
y-intercept=1/Vmax
x intercept=1/km
what does the dissociation constant equal
in MM Kd=Km=K-1/k1
lineweaver-burk equation
1/Vo=(km/vmax)(1/[S])+(1/vmax)
Card Set Information
Author:
Sheilaj
ID:
335007
Filename:
Enzyme Kinetics
Updated:
2017-10-19 16:24:28
Tags:
biochem
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Description:
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