Biochemistry Exam 1

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Author:
jdad
ID:
33509
Filename:
Biochemistry Exam 1
Updated:
2010-09-08 20:23:30
Tags:
Biochem Exam Review
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Description:
Eukaryotic Gene Expression
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  1. Evidence that rRNA is essential for Protein Synthesis
    Single cleavage in 16S rRNA could block translation

    23S rRNA essential for peptide bond formation

    Antibiotics interact with rRNA NOT protein
  2. RNA Polymerase Functions
    • Scans DNA for initiation sites
    • Unwinds short stretches of DNA (~17 bp)
    • Selects nucleotides
    • Catalyzes phosphodiester bond formation (5'-->3') at +1
    • Interacts with regualtory proteins to regulate transcription
  3. What are the ways to alter genes available for Transcription?
    • Methylation of DNA
    • Gene amplification
    • Gene rearrangement

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