Nucleic Acids

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The flashcards below were created by user Sheilaj on FreezingBlue Flashcards.


  1. base
    adenine (purine), guanine(purine), cytosine(pyrimidine), thymine(pyrimidine) ,uracil(pyrimidine)
  2. structural features of B-DNA
    • diameter 20 angstroms
    • Base pairs/ helical turn 10.5
    • helix rise/base pair 3.4 angstroms
    • right hand helix
  3. A and Z helix
    • Z: rare, left handed
    • A: crystallized form, right handed
  4. Sanger sequencing
    • need DNA polymerase, primer and template
    • Polymerase can either add ddntp or dntp
    • The vary locations of the ddnTps can determine fragment size
    • fragments run on gel
  5. Chemical Synthesis of DNA
    • Add DMT and attach 3' hydroxyl to Si resin
    • remove DMT
    • Add Nuc. with activating group and DMT
    • Oxidate to form triester
    • repeat until desired length formed
    • remove DMTĀ 
    • remove cyanoethyl groups from phosphates
    • cleave chain from silica support
  6. Types of DNA damage and consequences
    • Deamination
    • Depurination
    • Pyrimidine dimers
    • damaging agents
    • DNA mismatch
    • oxidative damage

Card Set Information

Author:
Sheilaj
ID:
335295
Filename:
Nucleic Acids
Updated:
2017-10-23 02:18:45
Tags:
Biochem
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Description:
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