Final-1-30

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what is science

the process of gaining knowledge and understanding about the natural world
What is the foundational assumption of science

forces and phenomena existing within the universe can be understood by applying a process of inquiry-the scientific method
Henderson-Hasselbalch equation

pH=pK-log[acid]/[base]
Understand what a binding constant (Kd) is and basic equation relating it to theta
- the lower the Kd the higher the affinity of the ligand for the protein
- theta=binding sites ocp./total binding sites=[L]/[L]+Kd
Michaelis-Menton Equation

V₀= Vmax[S]/Km+[S]
What is Km
- determines the affinity of an enzyme for its substrate
- lower Km higher affinity
Vmax

max rate of turnover
Kcat
- The turnover number – the number of substrate molecule each enzyme site converts to product per unit time
- Vmax/[Enzyme]
Effect of competitor inhibitor on enzyme kinetics
- Vo=Vmax[S]/Kmα+[S]
- inhibitor is mutually exclusive w/ S
- prevents binding S and vice versa
Uncompetitive inhibitor and mixed inhibitor
- uncomp-binds to the ES complex
- mixed- binds to both ES complex and E at a site distinct from that where S binds
Glycogen structure and consequences
- Glc(α1-->4)Glc
- granules
- insoluble
- branching (α1--->6)
Starch Structure consequences
- CHO storage in plants
- Glucose
- Amylose: (α1--->4), no branching
- Amylopectin:(α1--->4), branching
- helix
Cellulose structure and consequences
- fibrous structure in plants
- Glc(β1--->4)Glc
- linear
- Not Branched
DNA sequenced by the Sanger dideoxy method
- 3' template strand
- 5' primer + dNTPs + ddNTPs
- primer strand can add either d or ddNTPs
- generates fragments
- run on gel that separate by 1 nucleotide
- read from bottom top
relationship between UW, Vitamin D, and rickets
- Rickets is caused by Vitamin D deficiency
- Vitamin D content of food increases after exposure to UV light
- discovered at UW
Differences in rates of diffusion of lipids
- lateral diffusion of lipids is fast
- transverse diffusion slow
Identify which amino acids are likely to reside in a membrane

glycine, alanine, valine, leucine, isoleucine, proline, phenylalanine,tryptophan
passive facilitated diffusion
- passive transporters
- ex: glucose permease
- transporter action is enzyme-like
primary active transport

ATP- driven active transport
secondary active transport

gradient driven
Relation ship ΔG and ΔG'^o

ΔG= ΔG'o + RTln[D][C]/[A][B]
relationship between ΔG'o and Keq

ΔG'o=-RTlnKeq @ equilibirum
difference between kinetics and thermodynamics
- thermo-where the eq point
- kinetics-how fast is the eq point approached
7 common reactions in biochemistry
- Nucleophilic Substitution
- Aldol Codensation
- Claisen Condensation
- Decarboxylation of b-keto acids
- rearrangements or isomerizations
- elimination
- free radical reactions
result of phosphorylation

ADP and p-something
result of pyrophosphorylation

something-2Pi + AMP
result of adenylation

something-AMP + 2 Pi

Author

Sheilaj

336712

Card Set

Final-1-30

Description

stuff

Updated

2017-12-12T03:44:50Z

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