Micro Quiz 10/6/10
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Bacteria have multiple options for developing new traits
Mutation, Transformation, Conjugation, Transduction.
- Easier than in eukaryotes because of single chromosome
- Positive Selection
- Negative (indirect) selection
Rejects unmutated parental cells
- Replica Plating
- Negative (indirect selection)
- Method to identify potential carcinogens
- Most mutagens are carcinogenic
- Relies upon change in mutation rate
The flow of genetic information in bacteria
- Horizontal gene transfer: Between cells of the same generation. Can transfer DNA to its neighbor. It is usually the plasmid that is being shared.
- Vertical gene transfer: from one generation to the next
- Gene Expression: within a cell
Streptococis Pneumoniae. The one with a capsule (sugar coat) is virulent. A. immune system took care of Tye R nonvirulent. B. Type S virulent knew it would kill the mouse. C. boiled type S and inactivated it so it didn’t kill the mouse. D. Horizontal gene transfer. Contained Type R nonvirulent and boiled type S. Took up DNA from the dead ones and killed the mouse.
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