Home > Preview
The flashcards below were created by user
on FreezingBlue Flashcards.
Add DI water to slide, then add tiny amount of culture.Dry and heat-fix
Determine if Acid-fast or non acid-fast
- Carbolfuchsin (primary stain)
- Acid-alcohol (decolorizer)
- Methylene blue (counterstain or secondary stain)
Acid Fast Result: Red stained cells
Non-Acid Fast Result: Blue stained cells
Reagent: pH indicator phenol red
End-Product: Acid products (Yellow color) / gas (Bubble)Positive Reaction: Yellow color or yellow color with large gas bubbleNegative Reaction: Red to orange color / no gas bubble
Simmon’s citrate slant (bromthymol blue – pH indicator)
End Product: Alkaline (Base) product
What would you like to do?
Home > Flashcards > Print Preview