Microbio Lab

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faflovesmac
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41835
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Microbio Lab
Updated:
2010-10-28 12:08:11
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Microbiology Lab
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Study Cards for Lab Practical
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  1. Name the cellular morphology
    Coccomacillus
  2. Name the cellular morphology
    Spirochaetes
  3. Name the cellular morphology
    Tetrad
  4. Name the cellular morphology
    Diplococcus
  5. Name the cellular morphology
    Coccus/Cocci
  6. Name the cellular morphology
    Comma
  7. Name the cellular morphology
    Spirillum(a)
  8. Name the cellular morphology
    • staphylo-
    • i.e. Staphylococus aureus (grape-like clusters)
  9. Name the cellular morphology
    Strepto-
  10. Name the cellular morphology
    • Rods
    • i.e. Bacillus cereus (chains)
    • i.e. Escherichia coli (singles/pairs)
  11. Why is immersion oil important?
    • Immersion oil is important because there is no refraction of light when it passes from glass to oil and vice versa
    • Immersion oil results in clarity.
  12. Resolution
    Shortest distance between 2 points or objects whereby one can discern these 2 points/objects as separate entities
  13. Condenser
    Deals with intensity of light
  14. Iris diaphragm
    open & close to control light that comes in
  15. Par Focal
    As you move up in magnification, you should stay in focus
  16. TSA
    TSB (tryptic soy broth) + agar (sea kelp, no nutrients, solidifying agent)
  17. Colonial Morphology
    What the colony looks like
  18. Cellular Morphology
    What the cells look like
  19. TSB
    • Trypric Soy Broth
    • Medium
  20. Aseptic Transfer
    To work without contamination
  21. What kind of stain is this?
    Capsule stain
  22. What is the importance of a capsule stain? Name a microbe with a capsule
    • Tells us the presence or absence of a capsule
    • Klebsiella pneumoniae
  23. Name the flagellar arrangement
    Peritrichous
  24. Name the flagellar arrangement
    Amphitrichous
  25. Name the flagellar arrangement
    Lophotrichous
  26. Name the flagellar arrangement
    Monotrichous
  27. What is the importance of a flagellar stain? And give an example of a microbe with flagella
    • Tell us:
    • - Motility
    • - Arrangement

    Proteus mirabilis
  28. What is the importance of a spore stain?
    • A spore stain tells us:
    • - If its a spore former or not
    • - The position of the spore (terminal, subterminal, endospore, free spore)
  29. Name the image
    • terminal or subterminal spore
    • clostridium
  30. Name the image
    • Central spore
    • Bacillus
  31. List the steps of a spore stain
    • 1) Malachite green °1 - steam
    • 2) Safranin counterstain
  32. What is the importance of an acid fast stain
    • AFB red - Mycobacterium smegmatis
    • Non AFB blue - Escherichia coli
  33. List the steps of an Acid Fast stain
    • 1) Carbol Fushin - °1 steam
    • 2) Acidified ETOH - decolorize
    • 3) Methylene Blue - 2° or counterstain
  34. Give an example of Gram-
    • E. coli
    • Klebsiella pneumoniae
    • Ps. aeruginosa
    • S. marcesens
  35. Give an example of a Gram+
    • Bacillus (used in Gram stain and spore staining lab)
    • S. aureus (used in gram staining lab)
  36. List the steps of the Gram stain, and the outcomes for each step in a Gram+ and a Gram-
    • 1) Crystal violet °1 stain
    • 2) Gram's Iodine (mordant)
    • 3) Decolorize with 95% ETOH
    • 4) Safranin °2 (counterstain)

    • Gram+
    • 1) Purple
    • 2) Purple
    • 3) Purple
    • 4) Purple

    • Gram-
    • 1) Purple
    • 2) Purple
    • 3) Colorless
    • 4) Red
  37. Give examples of differential stains, and what are they used to identify?
    • Cell wall composition
    • - Acid fast
    • - Gram stain
    • Structure
    • - Spore
    • - Flagellar
    • - Capsule
  38. Why are differential stains used in lab?
    • Tells you the cell wall composition
    • Tells you the structure of the microbe
  39. What is a differential stain?
    Differential stains incorporate 2 or more stains
  40. Why are simple stains used in lab?
    • Tells you the presence or absence of a microbe
    • Tells you the cellular morphology
    • Tells you the arrangement
  41. What is a simple stain?
    A simple stain incorporates the use of one stain.
  42. What are the two staining techniques?
    • Simple
    • Differential
  43. What are the two types of stains in lab?
    • Basic
    • Acidic
  44. What is an acidic stain's overall charge?
    An acidic stain's have a negative overall charge.
  45. Give an example of an acidic stain
    An example of an acidic stain is Nigrosin.
  46. Why do we use acidic stains in lab?
    • We use acidic stains for the slide's background.
    • Since the slide has an overall positive charge, negative charges will bind to the glass.
  47. Why are basic stains used in lab?
    Because if we look at the surface of a bacterium, the cell wall has an overall negative charge.
  48. What is a basic stain's overall charge?
    Basic stains carry an overall positive charge.
  49. Name examples of basic stains
    • Crystal Violet
    • Safranin
    • Carbol Fushin
    • Methylene Blue
    • Malachite Green
  50. Colony
    group of microbes who have grown to a visible mass
  51. What is the main difference between a bacterial species that is "acid fast" and one that is "non-acid fast"?
    acid fast have mycolic acid
  52. Why do you use heat/steam when performing spore and acid fast stains?
    heat can facilitate the entrance of the dye
  53. generation time
    is the time interval required for the cells (or population) to divide.
  54. What is the generation time of a bacterial population that increases from 10,000 cells to 10,000,000 cells in four hours of growth
  55. aerobe
    • Bacillus
    • Pseudomonas aeruginosa
  56. Microaerophile
    lactococcus
  57. Facultative anaerobe
    e coli
  58. anaerobe
    clostridium
  59. acidophile
    saccharomyces cerevisiae
  60. neutrophile
    e. coli
  61. alkalophile
    alcaligenes faecalis
  62. psychrophile
    ps. fluorescens
  63. mesophile
    e coli
  64. thermophile
    bacillus stearothermophilus
  65. halotolerant
    s. aureus
  66. osmophile
    e. coli
  67. halophile
    • marine salt loving
    • vibrio alginolyticus

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