Describe how to make a synthetic peptide using Recombinant DNA methods.
Ingredients needed restriction endonucleases (used to cut open plasmid), desired gene, ligase, plasmid (vector), bacteria or yeast (cloning host). Add cDNA or DNA from other pathogen and plasmid that has been cut open with restriction endonucleases plus contain the gene for antibodic resistance, then ligase which ligates genes together then add to bacteria or yeast (cloning host) and the plasmids will stick to bacteria. It is then heat shocked which cause transformation (bacteria suck up DNA). Plate bacteria on agar with antibodics where they will grow up (only bacteria with gene will grow). These bacteria are used to grow large batches of bacteria with new gene that can be used to make a synthetic peptide.