SPE and SPME

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Author:
minna528
ID:
42421
Filename:
SPE and SPME
Updated:
2010-10-15 17:39:41
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Forensic chem
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Description:
Lecture 4
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  1. SPE stands for:
    Solid phase extraction
  2. In SPE analytes to be seperated are in a _______solution
    mobile phase
  3. Analytes are selectively partitioned on a _________ in SPE due to varying degrees of interaction with the stationary phase.
    Solid (stationary phase)
  4. The greater interaction during SPE the _______ analyte moves
    slower
  5. The less the interaction during SPE the _______ the analyte moves
    Faster
  6. When there is a large concentration of analyte interaction with the stationary phase the KD value is :
    Large
  7. when there is a small concentration of analyte interaction with the stationary phase the KD value is:
    Small
  8. What are the four major types of analyte-phase (mobile or stationary) interactions
    • Adsorption
    • Binding
    • Electrostatic
    • Solubility
  9. The stationary phase typically exists as:
    particles of silica
  10. Stationary phase particles have a ________ surface that increases available surface area to interact with analytes
    Porous
  11. Various______ are attached to the silica particles of the stationary phase
    R-groups
  12. What determines the affinity for particular classes of analytes on the stationary phase?
    The nature of the R-groups
  13. The mobile phase most commonly consists of:
    • Ethyl acetate
    • Methanol
    • Pentane
    • ect
  14. Reversed phase
    • Non-polar solid phase
    • polar solvents
  15. Normal phase
    • Polar solid phase
    • Non-polar solvents
  16. What are the four general steps of SPE
    • 1. Rinse column to condition
    • 2. Load sample
    • 3. Alter the pH of the system/Add Solvents
    • 4. Elute target analytes with different solvents and collect fractions

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