SPE and SPME
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SPE stands for:
Solid phase extraction
In SPE analytes to be seperated are in a _______solution
Analytes are selectively partitioned on a _________ in SPE due to varying degrees of interaction with the stationary phase.
Solid (stationary phase)
The greater interaction during SPE the _______ analyte moves
The less the interaction during SPE the _______ the analyte moves
When there is a large concentration of analyte interaction with the stationary phase the KD value is :
when there is a small concentration of analyte interaction with the stationary phase the KD value is:
What are the four major types of analyte-phase (mobile or stationary) interactions
The stationary phase typically exists as:
particles of silica
Stationary phase particles have a ________ surface that increases available surface area to interact with analytes
Various______ are attached to the silica particles of the stationary phase
What determines the affinity for particular classes of analytes on the stationary phase?
The nature of the R-groups
The mobile phase most commonly consists of:
- Ethyl acetate
- Non-polar solid phase
- polar solvents
- Polar solid phase
- Non-polar solvents
What are the four general steps of SPE
- 1. Rinse column to condition
- 2. Load sample
- 3. Alter the pH of the system/Add Solvents
- 4. Elute target analytes with different solvents and collect fractions
What would you like to do?
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