Chapter 6

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Chapter 6
2010-10-16 21:59:12
Cellular Molecular Biology

From gene to Protein
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  1. another word for mRNA
    hnRNA; heteronuclear RNA
  2. The job of U1 snRNP is?
    forms bade-pairs with 5' splice juntion and BBP (branch-point binding protein)and U2AF recognizes the branch-point site.
  3. How is the branch point site recognized?
    U2AF (U2 auxillary factor) recognizes the branch point
  4. What is a "spliceosome"?
    formed snRNP complex, utilizes 'A' at the branch site to cut the hnRNA molecule at the 5' splice site
  5. A ____ forms when an snRNP complex utilizes the branch site to ready the intron for splicing.
    a loop called a lariat
  6. What happens to the excised Intron lariat after it has been spliced out?
    intron RNA will be degraded in the nucleus; snRNPs will be recycled
  7. RNA polymerase __ transcribes RNA in the ___ to ___ direction.
    II; 5' to 3' direction
  8. ____ ____ is a sequence of DNA that transcribes
    trancriptional unit
  9. In eukaryotes, transcriptional unit inludes _ gene(s). In prokaryotes, a transcriptional unit includes _ gene(s).
    1 gene for eukaryotes; series of neighbouring genes for prokaryotes (think Lac operon)
  10. RNA polymerase can ____ the DNA helix at the active site.
    unwind the DNA to expose the template
  11. the ___ ___ signals the initiation phase for transcription on RNA.
    sigma factor; binds to RNA polymerase to create a haloenzyme.
  12. what sequence signals termination in transcription?
    ter sequences coded in the DNA cause RNA to form a loop; destablizes RNAs hold on DNA
  13. the ____ has affinity for RNA
  14. 2 important characterisitcs of ter sequences?
    • 1) sequences of DNA that codes for self-complimentary region; forms a loop in RNA to slow polymerase
    • 2) series of U-nucleotides in RNA make dissosiation easier
  15. TATAA box is located at the ___ region of the ____
    -10; promoter
  16. RNA polymerase always moves in the ___ to ___ direction and synthesizes RNA in the ____ to ____ direction.
    3' to 5'; 5' to 3'
  17. Eukaryotic transcription involves __ RNA polymerases
    3 RNA polymerases, RNA polymerase I,II, and III
  18. RNA polymerase I is composed of 3 subunits. Name them
    5.8s, 19s, and 28s . Codes for rRNA genes
  19. RNA polymerase II codes for? In eukaryotes.
    all-protein coding genes, snoRNA, miRNA, siRNA, and most snRNA
  20. RNA polymerase III codes for? (eukaryotes)
    tRNA, 5s rRNA genes, some sRNA genes and genes for other small RNAs
  21. True/false: Mitochondrial RNA polymerase & chloroplast RNA polymerase are both similar to bacterial RNA polymerase
  22. True/false: Not all RNA polymerases are composed of subunits.
    False. All RNA polymerases are composed of subunits.
  23. 3 groups of transcription factors required in transcription of eukaryotic genes?
    TFI, TFII, and TFIII