524 exam 2.6

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  1. what are neurotransmitters???
    • allows communication between cells at a synapse
    • typically occurs in 1 direction - from presynaptic to postsynaptic membrane
    • synthesized in presynaptic terminals, vesicles are recycled
    • released in response to action potential and influx of CALCIUM
    • released through EXOCYTOSIS
    • can initiate an excitatory postsynaptic potential (EPSP) or an inhibitory postsynaptic potential (IPSP)
  2. excitatory postsynaptic potential (EPSP)
    • depolarizes next neuron
    • excites next neuron
  3. inhibitory postsynaptic potential (IPSP)
    • hyperpolarizes next neuron
    • prevents NT release
  4. is effect on postsynaptic membrane ultimately determined by the receptor or the neurotransmitter?
    • RECEPTOR determines effect on postsynaptic membrane
    • some neurotransmitteres, depends on which receptor they bind to, can have an EPSP or IPSP.... therefore, can have different effects on the postsynaptic cell
  5. focus of this lecture? what are the 4 amino acid neurotransmitters? table 12.6
    • 1. glutamate
    • - mechanism of action: indirect - G proteins and 2nd messengers; direct - opens calcium channels on pre and post synaptic membranes
    • - important in memory and learning, most impt excitatory neurotransmitter in the brain
    • 2. aspartate
    • - mechanism of action: direct or indirect (G proteins, depending on type of receptor)
    • - used by pyramidal cells that provide voluntary motor control over skeletal muscles

    • 1. GABA - gamma aminobutyric acid
    • - mechanism of action: direct or indirect (G proteins, depending on type of receptor)
    • - dirrect effects: open chloride channels; indirect effects: open potassium channels and block entry of calcium
    • 2. glycine
    • - mechanism of action: direct - opens chloride channels
    • - produces postsynaptic inhibition; the poison strychnine produces fatal convulsions by blocking glycine receptors
  6. what are the 4 AA neurotransmitter size and class?
    • common neutrotransmitters are small molecules and work fast
    • they're approximately the size of 1 amino acid
    • less common neurotransmitters or neuromodulators vary in size and timing of effect, they modulate the activity of common NT (regulate the NT release)

    • the 4 AA NTs discussed in this lecture have about 10-19 atoms, and are the size of about 1 AA
    • glutamate, aspartate and glycine are AA themselves, act in CNS
    • GABA is an AA derivative, that also acts in the CNS
    • note!!! all 4 discussed in todays lecture only act in the CNS!!!!
  7. overview of the structure of the nervous system, where are the NTs released?
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    • 1. NTs from afferent neurons released into CNS
    • 2. NTs from interneurons released into CNS
    • 3. NTs from efferent neurons released into PNS (onto effector organ)
  8. distribution of NTs: specific pathways vs non specific pathways???
    • - some NTs only produced in specific areas of the CNS
    • - they're released in specific tracts in the CNS
    • - these are: seratonin, histamine, epi, norepi, dopa and ACh (biologic amines and ACh! beginning part of 12-6 graph)

    • - some NTs are widespread, not restricted to specific tracts
    • - the 4 AA discussed today have non specific pathways: glutamate, aspartate, GABA and glycine!!!
    • - glutamate is responsible for ~75% of excitatory transmission in the brain = GREEN LIGHT = major excitatory transmitter in the CNS!!!
    • - GABA os the NT at 20% of CNS synapses = RED LIGHT = major inhibitory transmitter in the CNS

    • so... just a reminder:
    • excitatory = glutamate + aspartate
    • inhibitory = GABA + glycine

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    note for GABA and glycine: K+ channel opened, K+ goes out of cell, cause hyperpolarization and not lead to an action potential... Cl- will go IN and cause hyperpolarization and cause IPSP
  10. RECEPTORS: ionotropic receptors vs metabotropic receptors
    also which one is faster?
    • - ligand gated ion channels
    • - associated with ion channels
    • - ligant (NT) receptor = ligand gated ion channel, opens and things move down gradient

    • - G protein coupled receptors (GPCRs)
    • - receptor associates with G protein which activates an ezyme which causes 2nd messenger... etc etc.

    ionotropic is faster, see an immediate response
  11. GLUTAMATE RECEPTORS: describe the 3 ionotropic receptors!!!
    all stimulates EPSPs!!!

    • 1. NMDA receptor (N-Methyl-D-Aspartate)
    • - when open, Na+ and Ca2+ enters the cell
    • - glycine is co-agonist (has to be around and bind as well as glutamate for this to work)
    • - also binds aspartate
    • - increase sodium and calcium influx, K+

    • 2. AMPA receptor
    • - mainly increases sodium, K+

    • 3. kainate receptor (seaweed)
    • - mainly increases sodium, K+
  12. GLUTAMATE RECEPTORS: describe the 1 metabotropic receptor
    • 1. GPCRs
    • - many subtypes identified
    • - most increase IP3 and intracellular calcium OR decrease cAMP
    • - stimulates EPSPs!!!
    • - remember Gs, Gi, and Gq... Gs and Gi leads to decrease cAMP, and Gq is for calcium increase
  13. interaction between glutamate receptors: NMDA and AMPA
    • - sustained by stimulation of AMPA Receptors leads to depolarization
    • - change in membrane potential activates NMDA receptor

    • - at resting membrane potentia, Mg2+ blocks the NMDA receptor from being permeable to ions... but depolarization allows Mg2+ to leave so ions can pass through
    • - soooo... need activated AMPA receptor first to cause a little bit of depolarization and take Mg2+ off NMDA receptor... then NMDA receptor to act
    • - glutamate around --> binds to AMPA --> Na+ enters the cell --> leads to a change in membrane potential --> depolarization --> Mg2+ can leave NMDA --> NMDA receptor can now bind to glutamate
    • - so basically a 2 step process just for NMDA to be activated
  14. why is the interaction between the 2 glutamate receptors impt? between NMDA and AMPA?
    • 1. synaptic plasticity: altering strength of the synapse
    • - can be altered by alterations in 2nd messenger pathways, kinases, phosphatases, receptor number, gene transcription

    2. LTP (long term potentiation): involved in memory and learning, so the more you repeat the pathway, the longer you retain the info, if you keep stimulating it, remember more
  15. glutamate reuptake
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    • glutamate is normally store in synaptic vesicles
    • they are released via calcium influx
    • reuptake of glutamate is done by NEURONS and ASTROCYTES to get recycled
    • 1. astrocytes convert glutamate --> glutamine
    • 2. glutamine is then transported to the neuron
    • 3. the neuron then converts glutamine to glutamate
    • 4. reuptake then stops the signal
  16. why is it impt for glutamate not to remain in synaptic cleft too long??? describe excess glutamate and excitotoxicity...
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    • when too much glutamate, actually toxic to neurons
    • when neuronal cell death occurs (due to stroke, exogenous exposure to ionotropic glutamate antagonist), glutamate doesn't undergo reuptae
    • this leads to excess glutamate available and excitotoxicity
    • neuronal death = glutamate released when cell dies (since its stored in the cell's vesicles) and can't be reabsorbed.. bec cell died!!!
  17. part 1 excess glutamate: describe what happens here: NMDA receptor vs non NMDA receptor
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    • NMDA receptor (left): the NMDA receptor is usually blocked by the Mg2+ ion... positive ions are unable to rush in even if glutamate binds to NMDA unless the Mg2+ ion is removed by an increase in the cell voltage (depolarization through AMPA receptor discussed earlier)
    • NON-NMDA receptor (right): the non-NMDA receptor opens as soon as glutamate binds to it... opening of the non-NMDA receptor allows the entry of positive ions into the cell
  18. part 2 excess glutamate: describe what happens here: calcium entry into the cell and cell death
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    • as glutamate continually binds to non-NMDA receptors and allows the entry of positive ions, the cell's voltage rises
    • ultimately, the voltage reaches a certain value that causes the Mg2+ ion to be removed from the opening of the NMDA receptor
    • calcium flows through the open NMDA receptor and causes various activities to occur that lead to cell death
    • causes: (1) increase in free radicals, (2) degrade cell membranes, (3) degrade proteins
    • so too much glutamate --> leads to activating NMDA receptors --> leads to too much calcium getting int o the cell --> leads to cell death!!!
    • and as described earlier... a little bit of cell death leads to more cell death!!!
  19. describe the 5 glutamate receptor drugs (NMDA channel blockers)
    • 1. Mg2+: endogenous
    • 2. ketamine: general anesthetic, dissociative anesthetic
    • 3. phencyclidine, specifically phecyclidine piperdine (PCP): effects include euphoria, hallucinations, hostility, violent behavior.. chronic use leads to memory loss and cognitive impairment
    • 4. methadone (top 200): has some affinity for NMDA receptor, but mainly an opoid antagonist which is used for opiate addiction (ex for heroine addiction) bec it binds to receptor but doesn't show effects of withdrawal
    • 5. memantine (NAMENDA, top 200): used to slow progression of alzheimer's (memory loss)... can prevent extra neuronal death and improve memory loss
  20. GABA (gamma-aminobutyric acid) RECEPTORS: describe the 2 receptors, 1 ionotrophic and 1 metabotrophic
    • 1. GABAA receptors (ionotrophic) - chloride channel, fast response, stimulates IPSPs
    • 2. GABAB receptors (metabotrophic, GPCR) - increase K+ influx OR decrease Ca2+ influx, slower response than GABAA, stimulates IPSPs
  21. describe the synthesis of GABA??? how is GABA made?
    • glutamate decarboxylase converts glutamate to GABA
    • glutamate decarboxylase only present in GABA-synthesizing neurons in CNS
  22. GABAA potentiation (how it increases nerve effect)
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    • - increases chloride influx
    • - increases inhibitory effects of GABA in the CNS
    • - this is because they bind to diff site, they can bind when GABA binds and increase inhibitory effect
    • - alcohol, bensodiazepines, barbituates and GABA all have different binding sites on receptor

    • 1. benzodiazepines (ends in -AM)
    • - increases RATE of channel opening
    • - used for bipolar disorder (manic episodes), anxiety disorder, panic disorder, insomnia
    • - anticonvulsant, antispasticity
    • - examples: alprazolam, lorazepam, diazepam

    • 2. barbituates
    • - increases DURATION of channel opening
    • - anticonvulsant, sedative/hypnotic
    • - example: phenobarbital (top 200)
  23. anxiety???
    • anxiety is he pervasive feeling of apprehension
    • basically helplessness, irritability, difficulty concentrating, insomnia
    • GI disturbances, muscle tension, tachycarida
  24. what are some anxiety disorders???
    • common symptoms include excessive fear of doom, loss of control , nervousness, dread... depression may accompany
    • examples: panic disorder, social anxiety disorder, generalized anxiety disorder, social phobia, specific phobias, OCD, posttraumatic stress syndrome
  25. what are some drugs used to treat anxiety disorders?
    • Rx: anxiolytics
    • 1. benzodiazepines... most commonly prescribed
    • 2. antidperessants, beta-adrenergic blockers
    • 3. buspirone... seratonin receptor agonist
  26. how do you treat insomnia???
    • basically... promote sleep via GABA potentiation
    • 1. benzodiazepines (top200): lorazepam and diazepam... inhibitory of other things, not just one pathway, so can make you sleepy
    • 2. non-benzodiazepines (top200): zolpidem (AMBIEN) and eszopiclone (LUNESTA)... subtype specific, litte tolerance or dependence
  27. describe presynaptic inhibition by GABA
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    • figure about shows how GABA alters activity of other NTs
    • if GABA is also released, it causes a decrease in the amount of calcium that enters the cell
    • GABA binds to the receptor to decrease the amount of calcium that goes in
    • if we decrease calcium influx, we decrease the amt of NTs released, and therefore, decrease the effect on the postsynaptic cell
    • we see that GABA is released from a separate neuron and acts on PREsynaptic cel
    • GABAB receptor alters calcium receptors
    • (rembember... GABAA is only for chloride, but B is for calcium and potassium)
  28. gamma hydroxybutyrate (GHB) endogenous source
    • endogenous source in CNS
    • has its own receptor and weakly binds to GABAB receptor (responsible for sedation) - IPSP
    • general sedative properties, addictive, and can cause wakefulness
    • makes you sleepy BUT if you do wake up abruptly, hard to fall asleep
  29. gamma hydroxybutyrate (GHB) synthetic drug
    • unlike natural GABA, GHB can cross the blood brain barrier
    • odorless, colorless liquid or powder, has a salty taste
    • also a product of fermentation, found in some beers and wines in small amounts
  30. gamma hydroxybutyrate (GHB) as a recreational drug
    • rave scene... basically called "liquid ecstacy" to reduce anxiety and create euphoria
    • bar scene... called "date rape" to induce stupor and reduce memory
    • gym scene... prolonged sleep causes increased growth hormone release
    • sometimes homebrewed from commercial agents... lethality due to bad homebrews (bec drain cleaner is involved) and excess does of drug is too inhibitory to neurons
  31. GLYCINE RECEPTOR: describe the only ionotropic glycine receptor
    • ionotripic
    • chloride channel
    • inhibitory effect IPSP
    • mostly affects spinal cord
  32. describe glycine inhibition: strychnine and tetanus toxin
    if we inhibit glycine, we inhibit the inhibitor... cause opposite effect... loose inhibition

    • 1. STRYCHNINE (from plant)
    • - BLOCKS glycine receptor
    • - rat poison
    • - loss of negative control
    • - causes convulsions and rigidity

    • 2. TETANUS TOXIN (from bacteria)
    • - INHIBITS glycine release
    • - promotes spasticity and tetanus
  33. REVIEW: what are the 4 AA Nt's and are they excitatory or inhibitory?
    • excitatory: glutamine and aspartate
    • inhibitory: glycine and gaba
  34. REVIEW: what are the 4 different types of glutamate receptors?
    • ionotropic: NMDA, AMPA, kainate
    • metabotropic glutamate receptor
  35. REVIEW: what is excitotoxicity?
    excess glutamate which leads to increase of calcium into the cell and leads to excess neuron cell death
  36. what regulates GABAA receptors?
    GABA, ethanol, barbituates and benzodiazepine
  37. what is GHB?
    • gamma hydroxy butyrate
    • weakly to GABAB + GHB receptor (its own receptor)
    • we have an endogenous source
    • date rape, liquid exstacy... etc.
  38. what two substances inhibit glycine receptors?
    strychnine and tetanus toxin

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524 exam 2.6
2010-10-24 23:54:56
exam dr bryant

524 exam 2.6 dr. bryant
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