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bkkrafft
on FreezingBlue Flashcards.
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- -ortho-tolidine
- -detects blood based on peroxidase-like activity of heme
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- - 1-Naphthyl Phosphate
- -component in Brentamine test for semen
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- 1-Naphtol
- -component in Brentamine test for semen
- -also in detection of sugars, Molisch test
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- Fast Blue B Salt
- -component of the Brentamine test for Semen
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Brentamine Test
- -detect the enzyme, acid phosphatase
- -presumptive screening for semen
1-naphthyl phosphate is added to sample. if acid phosphatase is present, 1-naphthol is formed. 1-naphthol reacts with the FBB salt to create a blue/purple color
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sodium rhodizionate
-detection of lead
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product of sodium rhodizionate test for lead
- - the test chelates lead in a pH dependent fashion
- - develops a red-violet color
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Sodium Rhodizionate Test
-detection of lead
An aqueous solution of Rhodizonic acid chelates lead in a pH dependent fashion. A red-violet color develops upon the addition of 5% HCl.
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rubeanic acid or dithiooxamide
- detection of copper and nickel
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copper rubeanate product from the dithiooxamide test for copper and nickel
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Dithiooxamide Test
- Dithiooxamide diluted with methanol and ammonium hydroxide is added to the sample.
- -olive green positive for copper
- -pinkish-blue positive for nickel
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diphenylamine
-detection of oxidizing salts
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Dectection of Oxidizing Salts
-diphenylamine and concentrated sulfuric acid are combined and then added to questioned samples.
blue is positive for oxidizing salts
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sulfanilic acid
Greiss #1
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N-(1-Naphthyl) Ethylenediamine dihydrochloride
Greiss #2
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The Greiss Test
-determines presence of nitrate and nitrite ions
- -add Greiss #1 followed by Greiss #2
- -pink/magenta positive for nitrite ions
- -nitrate ions remains colorless
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Detection of Sugars
The Molisch Test
1-Naphthol, ethanol, concentrated sulfuric acid
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