Thin Layer Chromatography

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Author:
bkkrafft
ID:
44394
Filename:
Thin Layer Chromatography
Updated:
2010-10-23 15:35:09
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BU Forensic Chemistry
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Description:
Lecture 4.1
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  1. _____ is a technique for separating mixtures into their components in order to analyze, identify, purify, and/or quantify the components of the mixture.
    Chromatography
  2. Chromatography separates mixtures into their components of a mixture by their distinctive attraction to what two phases?
    mobile and stationary
  3. Affinity
    natural attraction or force between things
  4. Mobile medium
    • Mobile phase
    • -gas or liquid that carries that components
  5. Stationary medium
    • Stationary phase
    • -the part of the apparatus that does not move with the sample
  6. T/F: Liquid chromatography separates dried liquid samples with a liquid solvent and a glass plate covered with a thin layer of alumina or silica gel.
    False! That is thin-layer chromatography!

    Liquid chromatography separates liquid samples with a liquid solvent and a column composed of solid beads.
  7. What type of chromatography separates vaporized samples with a carrier gas and a capillary column with an internal coating composed of polar or non-polar compounds?
    gas chromatography
  8. What are the four reasons why mixtures are separated into their component parts with chromatography?
    to analyze, purify, identify, and quantify
  9. In capillary action, what forces cause the movement of liquid within the spaces of the porous material?
    adhesion, cohesion, surface tension
  10. In TLC, when there is a Rf value closer to 1, the substances are _____ _____ in the liquid.
    very soluble
  11. In TLC, when there is a Rf value closer to 0, the substances are _____ _____ in the liquid.
    rather insoluble

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