SPE and SPME

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Author:
bkkrafft
ID:
44398
Filename:
SPE and SPME
Updated:
2010-10-23 16:06:48
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BU Forensic Chemistry
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Description:
Forensic Chemistry Lecture 4.2
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  1. What is the primary forensic application of Solid Phase Extraction?
    analysis of toxicology samples
  2. How are analytes separated in SPE?
    they are selectively partion on a solid due to varying degrees of interaction with the stationary phase
  3. T/F: In regards to SPE, as interaction of the analytes with the stationary phase increases, the slower the analytes move.
    True! The greater the interaction, the slower the analyte moves; the less interaction between them, the faster the analyte moves
  4. KD is the _______ coefficient.
    distribution
  5. When the KD value is large, is the concentration of analyte interaction with the stationary phase large or small?
    large
  6. When the KD value is small, is the concentration of analyte interaction with the stationary phase large or small?
    small
  7. What are the 4 major types of analyte-phase interactions?
    Adsorption, Binding, Electrostatic, Solubility
  8. T/F: In SPE, the reversed phase has a polar solid phase and non-polar solvents.
    False!! That is the normal phase. Reversed phase has non-polar solid phase and polar solvents.
  9. What are the primary forensic applications of Solid Phase MicroExtraction?
    analysis of controlled substances, ignitable liquids, and explosives/ explosive residues
  10. True or False: SPME is particularly useful for the extraction of volatile organic compounds.
    True!
  11. What are some of the disadvantages of SPME?
    • 1. It is ideally suited for highly volatile compounds and less volatile compounds require greater heating and longer desorption times.
    • 2. The solid coating of the fiber is thin and as a result the fiber is easily overloaded. When the fibers are saturated they cannot accept any more analyte.

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